PROPOLEOS Y HONGOS (FUNGUS)
| Items 1 - 47 of 47 |
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| 1: Acta Pharm. 2005 Dec;55(4):423-30. |
Flavonoid analysis
and antimicrobial activity of commercially available propolis products.
Kosalec
I, Pepeljnjak
S, Bakmaz
M, Vladimir-Knezevic
S.
Department of Microbiology, Faculty of Pharmacy and Biochemistry, University
of Zagreb, Zagreb, Croatia. ikosalec@pharma.hr
Propolis ethanolic solutions are the most used propolis products on the market
for the treatment of minor ulcers in the mouth, angina, thrush or skin infections.
Since it is still an unofficial drug in pharmacy, we analyzed the contents
of flavonoids in ten commercially available ethanolic solutions of propolis
from the Croatian market using two complementary colorimetric methods. Antimicrobial
activities, determined with the diffusion method, against six bacterial species
(Bacillus subtilis NCTC 8236, Staphylococcus aureus ATCC 25923, Streptococcus
pyogenes ATCC 12204, Enterococcus faecalis ATCC 29212, Escherichia coli ATCC
10536, Pseudomonas aeruginosa ATCC 27853, and one yeast-like fungus Candida
albicans ATCC 10231 were compared. Results of flavonoids analysis suggested
that the contents of flavones and flavonols in the products were uniform and
ranged from 0.14 to 0.41%, but the content of flavanones varied greatly from
0.43 to 18.78%. Total flavonoid content, as the sum of two colorimetric methods,
in propolis products was between 0.78 and 18.92%, and most products had the
flavonoids content below 9%. All products with the total flavonoids content
above 1% showed antimicrobial activity against the four Gram-positive bacterial
species tested, and against P. aeruginosa and the yeast-like fungus C. albicans.
Total flavonoids contents, expressed as the sum of two colorimetric methods,
could be useful methods for estimating the flavonoid contents of propolis
products. Our results indicate that the quality of commercially available
propolis products requires verification.
PMID: 16375832 [PubMed - in process]
| 2: Phytother Res. 2005 Jul;19(7):652-4. |
Oral candidiasis
treatment with Brazilian ethanol propolis extract.
Santos VR, Pimenta FJ, Aguiar MC, do Carmo MA,
Naves MD, Mesquita RA.
Department of Clinical Pathology
and Surgery, Laboratory of Microbiology, Dentistry School, Minas Gerais Federal
University, Campus da Pampulha, Belo Horizonte, Minas Gerais, Brazil, CEP
31.270-901. vegneer2003@yahoo.com.br
The Brazilian commercial ethanol propolis extract, also formulated to ensure
physical and chemical stability, was found to inhibit oral candidiasis in
12 denture-bearing patients with prosthesis stomatitis candidiasis association.
PMID: 16161031 [PubMed - indexed for MEDLINE]
| 3: Microbiol Res. 2005;160(2):189-95. |
Chemical compositions
and antimicrobial activities of four different Anatolian propolis samples.
Uzel A, Sorkun K, Oncag O, Cogulu D, Gencay O, Salih B.
Department of Biology, Faculty
of Sciences, Ege University, Basic and Industrial Microbiology Section, Bornova-Izmir
35100, Turkey. uzel@sci.ege.edu.tr
Propolis means a gum that is gathered by bees from various plants. It is known
for its biological properties, having antibacterial, antifungal and healing
properties. The aims of this study were to evaluate the antimicrobial activity
of four different Anatolian propolis samples on different groups of microorganisms
including some oral pathogens and comparison between their chemical compositions.
Ethanol extracts of propolis (EEP) were prepared from four different Anatolian
propolis samples and examined whether EEP inhibit the growth of the test microorganisms
or not. For the antimicrobial activity assays, minimum inhibitory concentrations
(MIC) were determined by using macrodilution method. The MIC values of the
most effective propolis (TB) were 2 microg/ml for Streptococcus sobrinus and
Enterococcus faecalis, 4 microg/ml for Micrococcus luteus, Candida albicans
and C. krusei, 8 microg/ml for Streptococcus mutans, Staphylococcus aureus,
Staphylococcus epidermidis and Enterobacter aerogenes, 16 microg/ml for Escherichia
coli and C. tropicalis and 32 microg/ml for Salmonella typhimurium and Pseudomonas
aeruginosa. The chemical compositions of EEP's were determined by high-temperature
high-resolution gas chromatography coupled to mass spectrometry. The main
compounds of four Anatolian propolis samples were flavonoids such as pinocembrin,
pinostropin, isalpinin, pinobanksin, quercetin, naringenin, galangine and
chrysin. Although propolis samples were collected from different regions of
Anatolia all showed significant antimicrobial activity against the Gram positive
bacteria and yeasts. Propolis can prevent dental caries since it demonstrated
significant antimicrobial activity against the microorganisms such as Streptococcus
mutans, Streptococcus sobrinus and C. albicans, which involves in oral diseases.
PMID: 15881836 [PubMed - indexed for MEDLINE]
| 4: J Ethnopharmacol. 2005 May 13;99(1):69-73. |
Chemical
composition and antibacterial activity of propolis collected by three different
races of honeybees in the same region.
Silici
S, Kutluca
S.
Erciyes University, S.Cikrikcioglu Vocational College, Department of Beekeeping,
38039 Kayseri, Turkey. silicis@erciyes.edu.tr
The chemical analysis and antibacterial activity of three types of propolis
collected three different races of Apis mellifera bee in the same apiary were
investigated. Propolis samples were investigated by GC/MS, 48 compounds were
identified 32 being new for propolis. The compounds identified indicated that
the main plant sources of propolis were Populus alba, Populus tremuloides
and Salix alba. The antimicrobial activity against Staphylococcus aureus,
Escherichia coli, Pseudomonas aeruginosa and Candida albicans was evaluated.
Ethanolic extracts of propolis samples showed high antibacterial activity
against Gram-positive cocci (Staphylococcus aureus), but had a weak activity
against Gram-negative bacteria (Escherichia coli and Pseudomonas aeruginosa)
and yeast (Candida albicans). Propolis sample collected by Apis mellifera
caucasica showed a higher antibacterial activity than collected by Apis mellifera
anatolica and Apis mellifera carnica.
PMID: 15848022 [PubMed - indexed for MEDLINE]
| 5: Mycoses. 2005 May;48(3):205-10. |
Comparison
of in vitro activities of antifungal drugs and ethanolic extract of propolis
against Trichophyton rubrum and T. mentagrophytes by using a microdilution
assay.
Koc
AN, Silici
S, Ayangil
D, Ferahbas
A, Cankaya
S.
Medical Faculty, Department of Microbiology, Erciyes University, Kayseri,
Turkey.
The in vitro activities of propolis against 29 strains of dermatophytes were
compared with those of terbinafine, itraconazole, ketoconazole, and fluconazole.
Minimal inhibitory concentrations (MICs) were determined according to a National
Committee for Clinical Laboratory Standards broth microdilution method. Among
the systemic antifungals tested, terbinafine was the most potent. Propolis
showed important antifungal activity and it merits further investigation as
a potentially useful agent for the treatment of dermatophytosis.
PMID: 15842339 [PubMed - indexed for MEDLINE]
| 6: Mikrobiol Z. 2004 Nov-Dec;66(6):53-7. |
[Antimicrobial properties
of bee preparations in ointment form]
[Article in Ukrainian]
Postoienko
VO, Senchuhova
NA, Postoienko
OM, Patyka
VP.
High antimicrobial activity apiphytopreparation in the form of the ointments
containing pine turpentine, different concentration of propolis (from 4 to
20 %), honey and carotene oil from carrots (ointment N 4) has been. While
testing by the method of diffusion in agar all the studied apiphytopreparation
suppressed growth of Staphylococcus aureus, Bacillus subtilis, Saccharomyces
cerevisiae, Micrococcus luteus, M. roseus. This effect increased with propolis
content increase in the content of ointments. Bee honey and carotene oil intensified
their antimicrobial activity. The latter was caused by the action of phenolic
compounds, ether oils and other biologically active substances--the apiculture
and vegetative raw material.
PMID: 15765871 [PubMed - indexed for MEDLINE]
| 7: Oral Health Prev Dent. 2003;1(1):29-35. |
Propolis extract
as an adjuvant to periodontal treatment.
Gebaraa
EC, Pustiglioni
AN, de Lima
LA, Mayer
MP.
Department of Microbiology, Institute of Biomedical Sciences, University of
Sao Paulo, Sao Paulo, Brazil. gebar@uol.com.br
PURPOSE: The aim of this study was to evaluate the effect of subgingival irrigation
with propolis extract by clinical and microbiological parameters. MATERIALS
AND METHODS: Twenty patients diagnosed with chronic periodontitis presenting
three non-adjacent teeth with deep pockets were selected. After scaling and
root planing, the selected periodontal sites were submitted to one of the
following treatments: irrigation with a hydro alcoholic solution of propolis
extract twice/week for two weeks (group A); irrigation with a placebo twice/week
for two weeks (group B); or no additional treatment (C). Subgingival plaque
sampling and scaling and root planing were performed two weeks after clinical
data recording. Two weeks later irrigation procedures were started (Baseline).
Microbiological and clinical data were collected at baseline, and after 4,
6 and 24 weeks. RESULTS: A decrease in total viable counts of anaerobic bacteria
(p=0.007), an increase in the proportion of sites with low levels (< or
= 10(3) cfu/mL) of Porphyromonas gingivalis (p=0.005), and a decrease in the
number of sites with detectable presence of yeasts (p=0.000) were observed
in group A sites when compared to group B and C sites. Propolis treatment
did not lead to an increase in organisms such as coagulase positive Staphylococci
and Pseudomonas spp. 24 weeks after treatment there was an increased proportion
of sites showing probing depth (PD) < or =
| 8: Nahrung. 2004 Jun;48(3):188-94. |
Inhibitory effect
of pollen and propolis extracts.
Ozcan
M, Unver
A, Ceylan
DA, Yetisir
R.
Department of Food Engineering, Faculty of Agriculture, Selcuk University,
Konya, Turkey. mozcan@selcuk.edu.tr
Bee pollen and propolis were collected from Apis mellifera colonies in five
regions of Turkey. The antifungal properties of methanol extracts of pollen
and propolis (2% and 5% concentrations) were determined on Alternaria alternata
and Fusarium oxysporium f. sp. melonis. The least active concentration towards
the tested fungi was 2% concentration of both extracts. The inhibitory effect
of all propolis extracts on growth of F. oxysporium and A. alternata were
generally higher when compared with pollen extracts. The growth of A. alternata
and F. oxysporium were not affected at both concentrations of pollens. However,
F. oxysporium against propolis extracts was more sensitive than A. alternata
(P < 0.01). None of the pollen extracts tested completely inhibited mycelial
growth of fungi used in our experiment. Percent inhibition of both pollen
concentrations against A. alternata and F. oxysporium was lower than 50%.
However, both concentrations of Alanya and Beysehir propolis extracts were
100% effective on mycelial growth of F. oxysporium until the 7th day of incubation
(P < 0.01). 2% Alanya and Beysehir pollen extracts completely stimulated
mycelial growth of F. oxysporium on the 7th day of incubation. Both concentrations
of propolis extract showed more than 50% inhibition against E. oxysporium.
It is suggested that high concentrations ofpropolis extract could be used
as an antifungal agent against tested fungi.
PMID: 15285109 [PubMed - indexed for MEDLINE]
| 9: Planta Med. 2004 Jun;70(6):515-9. |
Chemical
analysis and antimicrobial activity of Greek propolis.
Melliou
E, Chinou
I.
Division of Pharmacognosy and Natural Products Chemistry, Department of Pharmacy,
University of Athens, 157-71 Athens, Greece.
One new 2,3-dihydroflavone derivative, 7- O-prenylstrobopinin, and 25 known
diterpenes and phenolic compounds were identified from the n-butanol extract
of Greek propolis. This is the first time that diterpenes have been isolated
from propolis of European origin, while six of the known compounds are reported
as propolis constituents for the first time. The structures of the isolated
compounds were determined by spectroscopic methods, mainly by the concerted
application of 1D, 2D NMR techniques (HMQC, HMBC, NOESY) and mass spectrometry.
The studied sample and the isolated compounds were tested for their antimicrobial
activity against Gram (+/-) bacteria and fungi and five of them exhibited
strong activities.
PMID: 15229802 [PubMed - indexed for MEDLINE]
| 10: J Med Food. 2004 Spring;7(1):114-6. |
Inhibition
of Aspergillus parasiticus NRRL 2999 by pollen and propolis extracts.
Ozcan
M.
Department of Food Engineering, Faculty of Agriculture, Selcuk University,
Konya, Turkey. mozcan@selcuk.edu.tr
The inhibitory effects on mycelial growth of Aspergillus parasiticus strain
NRRL 2998 of pollen and propolis extracts from different regions of Turkey
were investigated in culture media. The least active concentration towards
the tested fungus was 2% of both extracts. But, the inhibitory effects of
all propolis extracts on mycelial growth were higher when compared with pollen
extracts. While the effect of a 5% level of Antakya propolis extract increased
with increasing incubation period, the antifungal effect of pollen extract
from the same region on mycelial growth was less than that of the control
group. As a result, none of the extracts tested completely inhibited mycelial
growth. The highest inhibition rate was established at the 5% level of Taskent
and Alanya propolis samples.
PMID: 15117563 [PubMed - indexed for MEDLINE]
| 11: J Med Food. 2004 Spring;7(1):90-4. |
An in vitro
study on antimicrobial activity of propolis from Mugla province of Turkey.
Ugur
A, Arslan
T.
Department of Biology, Faculty of Arts and Sciences, Mugla University, Kotekli-Mugla,
Turkey. augur@mu.edu.tr
Antibacterial and antifungal activities of acetone and dimethyl sulfoxide
(DMSO) extracts of 45 different propolis samples from the Mugla province of
Turkey were investigated. Antimicrobial activity of propolis varied depending
on propolis sample, dosage of propolis, and the extraction solvents for all
test microorganisms. Antimicrobial activity of all propolis samples increased
with increasing dosage without reaching a plateau at the highest dosage tested.
Except for Brucella melitensis, the DMSO extracts of all propolis samples
were more active than the acetone extracts of the same samples. For B. melitensis,
the acetone extracts of all propolis samples showed greater activity. The
most sensitive microorganism to propolis was Shigella sonnei in the gram-negative
group and Streptococcus mutans in the gram-positive group. The least sensitive
microorganism was Candida albicans. A control test run with standard antibiotics
revealed that propolis samples from the Mugla province of Turkey has a similar
or greater inhibitory effect on S. mutans, Salmonella typhi, Pseudomonas aeruginosa,
S. sonnei, and C. albicans growth.
PMID: 15117559 [PubMed - indexed for MEDLINE]
| 12: Lett Appl Microbiol. 2004;38(2):87-92. |
Chemical
composition and microbicidal activity of extracts from Brazilian and Bulgarian
propolis.
Salomao K, Dantas AP, Borba CM, Campos LC, Machado DG, Aquino Neto FR,
de Castro SL.
Dept. de Ultra-estrutura e Biologia
Celular Dept. de Micologia Dept. de Bacteriologia, Instituto Oswaldo Cruz,
FIOCRUZ, Rio de Janeiro, Brazil. solange@ioc.fiocruz.br
AIMS: The chemical composition of ethanol extracts from a Brazilian (Et-Bra)
and a Bulgarian (Et-Blg) propolis, and their activity against the protozoan
Trypanosoma cruzi, several fungi and bacteria species were determined. METHODS
AND RESULTS: The chemical composition was determined by high temperature high
resolution gas chromatography coupled to mass spectrometry. Microbiological
activity was assayed in vitro against T. cruzi, Candida albicans, Sporothrix
schenckii, Paracoccidioides brasiliensis, Neisseria meningitidis, Streptococcus
pneumoniae and Staphylococcus aureus. CONCLUSIONS: Et-Bra and Et-Blg, although
with totally distinct compositions, were active against T. cruzi and the three
species of fungi. Et-Blg was more effective than Et-Bra against bacteria,
particularly N. meningitidis and Strep. pneumoniae. SIGNIFICANCE AND IMPACT
OF THE STUDY: Although with different classes of components, both propolis
extracts showed microbicidal activity. For the bactericidal activity it was
possible to establish a positive correlation with the high content of flavonoids
of the Bulgarian extract.
PMID: 14746537 [PubMed - indexed for MEDLINE]
| 13: Microbiol Res. 2003;158(4):353-7. |
In vitro antimicrobial
activity of propolis and synergism between propolis and antimicrobial drugs.
Stepanovic
S, Antic
N, Dakic
I, Svabic-Vlahovic
M.
Department of Bacteriology, Institute of Microbiology and Immunology, School
of Medicine, University of Belgrade, Dr Subotica 1, 11000 Belgrade, Serbia.
stepan@afrodita.rcub.bg.ac.yu
The aim of this study was to investigate antimicrobial properties of ethanolic
extract of 13 propolis (EEP) samples from different regions of Serbia against
39 microorganisms (14 resistant or multiresistant to antibiotics), and to
determine synergistic activity between antimicrobials and propolis. Antimicrobial
activity of propolis samples was evaluated by agar diffusion and agar dilution
method. The synergistic action of propolis with antimicrobial drugs was assayed
by the disc diffusion method on agar containing subinhibitory concentrations
of propolis. Obtained results indicate that EEP, irrespectively of microbial
resistance to antibiotics, showed significant antimicrobial activities against
Gram-positive bacteria (MIC 0.078%-1.25% of EEP) and yeasts (0.16%-1.25%),
while Gram-negative bacteria were less susceptible (1.25%-->5%). Enterococcus
faecalis was the most resistant Gram-positive bacterium, Salmonella spp. the
most resistant Gram-negative bacteria, and Candida albicans the most resistant
yeast. EEP showed synergism with selected antibiotics, and displayed ability
to enhance the activities of antifungals. The shown antimicrobial potential
of propolis alone or in combination with certain antibiotics and antifungals
is of potential medical interest.
PMID: 14717457 [PubMed - indexed for MEDLINE]
| 14: Mutat Res. 2003 Nov;544(2-3):195-201. |
Dietary components
may prevent mutation-related diseases in humans.
Ribeiro LR, Salvadori DM.
Programa de Pos-Graduacao
Publication Types:
· Review
PMID: 14644321 [PubMed - indexed for MEDLINE]
| 15: J Chemother. 2003 Oct;15(5):454-60. |
Effect of propolis
on virulence factors of Candida albicans.
D'Auria
FD, Tecca
M, Scazzocchio
F, Renzini
V, Strippoli
V.
Institute of Microbiology, Faculty of Pharmacy, University of Rome La Sapienza,
P.le A.Moro, 5, 00185 Rome, Italy. felicia.d'auria@uniroma1.it
Propolis is a resinous substance collected by honeybees from plant sources.
Its antimicrobial activity has been well documented but little is specifically
known about its activity on virulence factors of Candida albicans. The aim
of this work was therefore to evaluate in vitro the propolis effect on yeast-mycelial
conversion (Y-M), extracellular phospholipase activity and fungal adhesion
to epithelial cells. The two propolis samples used significantly inhibited
the C. albicans strains tested, showing a rapid (between 30 seconds and 15
minutes), dose-dependent cytocidal activity and an inhibitory effect on Y-M
conversion at a concentration of 0.22 mg/ml. Moreover, the hyphal length was
reduced even at lower propolis concentration. Propolis also caused a dose-
and time-dependent inhibition of phospholipase activity. No clear effect was
shown on adherence to buccal epithelial cells and surface structure hydrophobicity,
but damage to the plasma membrane structure was demonstrated with the Propidium
Iodide test.
PMID: 14598937 [PubMed - indexed for MEDLINE]
| 16: J Ethnopharmacol. 2003 Oct;88(2-3):189-93. |
Flavonoids
and trypanocidal activity of Bulgarian propolis.
Prytzyk E, Dantas AP, Salomao K, Pereira AS, Bankova VS, De Castro SL,
Neto FR.
Ladetec, Instituto de Quimica, Universidade Federal do Rio de Janeiro, Ilha
do Fundao, Cidade Universitaria, CT Bl. A, 21949-900 Rio de Janeiro, RJ, Brazil.
Acetone and ethanol extracts of two Bulgarian propolis samples (Bur and Lov)
were investigated by high temperature high resolution gas chromatography coupled
to mass spectrometry (HT-HRGC-MS), and their activity against Trypanosoma
cruzi was evaluated. The ethanol extracts--Et-Bur and Et-Lov--showed similar
composition, with a high content of flavonoids, and strong inhibitory activity
against T. cruzi proliferative epimastigotes, which were more susceptible
than trypomastigotes. In the presence of blood, the activity of Et-Bur or
Et-Lov against trypomastigotes was similar to that of the standard drug, crystal
violet. Both extracts also showed similar and significant activity against
Staphylococcus aureus and Candida albicans, while being inactive against Escherichia
coli. The acetone extract, Ket-Bur, was more active than Et-Bur against both
forms of T. cruzi.
PMID: 12963141 [PubMed - indexed for MEDLINE]
| 17: J Ethnopharmacol. 2003 May;86(1):69-73. |
Antimicrobial
activity of propolis samples from two different regions of Anatolia.
Kartal
M, Yildiz
S, Kaya
S, Kurucu
S, Topcu
G.
Department of Pharmacognosy, Faculty of Pharmacy, Ankara University, 06100
Ankara, Turkey. kartal@pharmacy.ankara.edu.tr
Antimicrobial activity of two propolis samples from Kazan and Marmaris regions
in Turkey were investigated by the disc diffusion method. Antimicrobial activity
was tested with four different ethanolic extracts (30, 50, 70, and 96% ethanol)
of each sample against seven Gram positive, four Gram negative bacteria and
one fungus culture. The activity was found to be mainly due to caffeic acid
and its esters. An isomeric mixture containing 3,3-dimethylallyl caffeate,
and isopent-3-enyl caffeate was isolated from Kazan propolis samples.
PMID: 12686444 [PubMed - indexed for MEDLINE]
| 18: Int J Syst Evol Microbiol. 2003 Jan;53(Pt 1):339-43. |
Starmerella
meliponinorum sp. nov., a novel ascomycetous yeast species associated with
stingless bees.
Teixeira AC,
Marini MM, Nicoli JR, Antonini Y, Martins RP, Lachance MA,
Rosa CA.
Departamento de Microbiologia, ICB, CP 486, Universidade Federal de Minas
Gerais, Belo Horizonte, MG, 31270-901, Brazil.
Thirty-two strains of the novel
species Starmerella meliponinorum sp. nov. were isolated from various substrates
associated with three stingless bee species (tribe Meliponini) in Brazil and
one in Costa Rica. The strains were found in garbage pellets (faecal material,
discarded pollen or food, etc.), pollen provisions, adult bees, honey and
propolis of Tetragonisca angustula, in honey from Melipona quadritasciata
and in adults of Melipona rufiventris and Trigona fulviventris. The sequence
of the D1/D2 domains of the large-subunit rDNA showed that the novel species
belongs to the Starmerella clade and is most closely related to Candida etchellsii,
although the two differ in their sequences by 7% base substitutions. S. meliponinorum
is homothallic and assimilates few carbon sources. Nitrate is utilized as
a sole nitrogen source. The type strain of S. meliponinorum is strain UFMG-01-J26.1T
(=CBS 9117T).
PMID: 12656193 [PubMed - indexed for MEDLINE]
| 19: Lett Appl Microbiol. 2002;35(3):203-7. |
Comparative
study of in vitro methods used to analyse the activity of propolis extracts
with different compositions against species of Candida.
Sawaya AC, Palma AM, Caetano FM, Marcucci MC,
da Silva Cunha IB,
Araujo CE, Shimizu MT.
Universidade Sao Francisco, Centro de Ciencias Biologicas e da Saude, Braganca
Paulista, SP, Brazil.
AIMS: Propolis is known for its
activity against micro-organisms and different in vitro assays have been used
to evaluate this activity, frequently with contradictory results. METHODS
AND RESULTS: Brazilian propolis from the state of Sao Paulo was extracted
by maceration using different concentrations of ethanol and water. The resultant
extracts were analysed by chromatographic methods. Several microbiological
methods were compared to determine which one best evaluated the activity of
the propolis extracts against species of Candida, with average minimal inhibitory
concentration values between 6 and 12 mg ml(-1). CONCLUSIONS: Agar dilution
in plates showed the clearest results. These were in agreement with the chromatographic
analyses, which also identified the active substances. SIGNIFICANCE AND IMPACT
OF THE STUDY: Although the active substances identified in this sample are
typical of Brazilian propolis, their activity against Candida had not been
recognized previously, demonstrating the importance of standardizing the correct
combination of microbiological and chromatographic analyses.
Publication Types:
PMID: 12180941 [PubMed - indexed for MEDLINE]
| 20: Z Naturforsch [C]. 2002 Mar-Apr;57(3-4):395-402. |
Egyptian propolis:
3. Antioxidant, antimicrobial activities and chemical composition of propolis
from reclaimed lands.
Hegazi
AG, Abd
El Hady FK.
Department of Parasitology, National Research Center, Dokki, Giza, Egypt.
ahmedgaffer@mailer.suc.eun.eg
The free radical scavenging effect of two propolis samples collected from
reclaimed land, Egypt as well as of vitamin C and caffeic acid in 1,1-diphenyl-2-picrylhydrazyl
(DPPH) free radical system was determined. The antimicrobial (Staphylococcus
aureus; Escherichia coli and Candida albicans) activity was also investigated.
The results of the free radical scavenging effect of El-Saff and Ismailia
propolis showed a concentration-dependent activity. The antioxidant activity
was varied according to the examined material. It was obvious that caffeic
acid and vitamin C showed the highest activity if compared with the propolis
samples. El- Saff propolis had a higher antioxidant activity than Ismailia
propolis, it showed a higher antibacterial activity against Staphylococcus
aureus and a higher anti-fungal activity against Candida albicans. While the
Ismailia propolis had a higher antibacterial activity against Escherichia
coli, than El-Saff propolis. The chemical composition of propolis samples
was investigated by GC/MS, where 75 compounds were identified, 22 being new
for propolis. The Ismailia propolis was characterized by the presence of a
highly significant amount of aromatic acid esters (47.3%) and triterpenoids
(17.3%), while El-Saff propolis contained 3% and 1.9% respectively. The new
esters belonged to 4-methoxyhydrocinnamic acid, hydroferulic acid and ferulic
acid. El-Saff propolis had a very high significant amount (27%) of 2,6-bis-(pentanyloxy)-4-pentanylphenethanol,
which is also a new compound for propolis.
PMID: 12064746 [PubMed - indexed for MEDLINE]
| 21: Z Naturforsch [C]. 2002 Mar-Apr;57(3-4):386-94. |
Egyptian propolis:
2. Chemical composition, antiviral and antimicrobial activities of East Nile
Delta propolis.
Abd
El Hady FK, Hegazi
AG.
Department of Chemistry of Natural Products, National Research Center, Dokki,
Giza, Egypt.
Three propolis samples from East Nile Delta, Egypt were collected. Propolis
samples were investigated by GC/MS,103 compounds were identified, 20 being
new for propolis. Dakahlia propolis was a typical poplar propolis but it contained
two new caffeate esters and two new triterpenoids. Ismailia propolis was characterized
by the presence of new triterpenic acid methyl esters and it did not contain
any aromatic acids, esters and flavonoids. Sharkia propolis was characterized
by the presence of caffeate esters only, some di- and triterpenoids. The antiviral
(Infectious Bursal Disease Virus and Reo-Virus) and antimicrobial (Staphylococcus
aureus; Escherichia coli and Candida albicans) activities of propolis samples
were investigated. Dakahlia propolis showed the highest antiviral activity
against Infectious Bursal Disease Virus (IBDV) and the highest antibacterial
activity against Escherichia coli and the highest antifungal activity against
Candida albicans. While Ismailia propolis had the highest antiviral activity
against Reo-virus. Sharkia propolis showed the highest antibacterial activity
against Staphylococcus aureus and moderate antiviral activity against infectious
bursal disease virus and reovirus.
PMID: 12064745 [PubMed - indexed for MEDLINE]
| 22: J Oral Sci. 2002 Mar;44(1):41-8. |
Effect of commercial
ethanol propolis extract on the in vitro growth of Candida albicans collected
from HIV-seropositive and HIV-seronegative Brazilian patients with oral candidiasis.
Martins RS, Pereira ES Jr,
Lima SM, Senna MI, Mesquita RA,
Santos VR.
Department of Clinical Pathology
and Surgery, School of Dentistry, Minas Gerais Federal University, Belo Horizonte,
Brazil.
The present study assessed the susceptibility of Candida albicans strains,
collected from HIV-positive patients with oral candidiasis, to a commercial
20% ethanol propolis extract (EPE) and compare it to the inhibitory action
of the standardized antifungal agents nystatin (NYS), clotrimazole (CL), econazole
(EC), and fluconazole (FL). Twelve C. albicans strains collected from HIV-positive
patients with oral candidiasis were tested. The inhibition zones were measured
with a pachimeter and the results are reported as means and standard deviation
(M +/- SD). Data were analyzed statistically by the non-parametric Kruskal-Wallis
test. EPE inhibited all the C. albicans strained tested. No significant difference
was observed between the results obtained with NYS and EPE, while significant
differences were observed between EPE and other antifungals. The C. albicans
strains tested showed resistance to the remaining antifungal agents. The propolis
extract used in this study inhibited the in vitro growth of C. albicans collected
from HIV-seropositive Brazilian patients, creating/forming inhibition zones
like those ones formed by NYS. This fact suggests that commercial EPE could
be an alternative medicine in the treatment of candidiasis from HIV-positive
patients. However, in vivo studies of the effect of EPE are needed to determine
its possible effects on the oral mucosa.
PMID: 12058869 [PubMed - indexed for MEDLINE]
| 23: J Ethnopharmacol. 2002 Mar;79(3):331-4. |
Effects of
propolis from Brazil and Bulgaria on fungicidal activity of macrophages against
Paracoccidioides brasiliensis.
Murad
JM, Calvi
SA, Soares
AM, Bankova
V, Sforcin
JM.
Department of Microbiology and Immunology, Biosciences Institute, IB-UNESP,
18618-000 Botucatu, SP, Brazil
Paracoccidioidomycosis is the most important systemic mycosis in Latin America.
Its etiological agent, Paracoccidoides brasiliensis, affects individuals living
in endemic areas through inhalation of airborne conidia or mycelial fragments.
The disease may affect different organs and systems, with multiple clinical
features, with cell-mediated immunity playing a significant role in host defence.
Peritoneal macrophages from BALB/c mice were stimulated with Brazilian or
Bulgarian propolis and subsequently challenged with P. brasiliensis. Data
suggest an increase in the fungicidal activity of macrophages by propolis
stimulation, independently from its geographic origin.
PMID: 11849837 [PubMed - indexed for MEDLINE]
| 24: Mycoses. 2001 Nov;44(9-10):375-8. |
Antifungal
activity of propolis on different species of Candida.
Ota C, Unterkircher C,
Fantinato V,
Shimizu MT.
Dental School, UNESP, Sao Jose dos Campos, SP, Brasil.
Propolis is a resinous material collected by bees from the buds or other parts
of plants. It is known for its biological properties, having antibacterial,
antifungal and healing properties. The antifungal activity of propolis was
studied in sensitivity tests on 80 strains of Candida yeasts: 20 strains of
Candida albicans, 20 strains of Candida tropicalis, 20 strains of Candida
krusei and 15 strains of Candida guilliermondii. The yeasts showed a clear
antifungal activity with the following order of sensitivity: C. albicans >
C. tropicalis > C. krusei > C. guilliermondii. Patients with full dentures
who used a hydroalcoholic propolis extract showed a decrease in the number
of Candida.
PMID: 11766101 [PubMed - indexed for MEDLINE]
| 25: Z Naturforsch [C]. 2001 Jul-Aug;56(7-8):593-6. |
New bioactive chalcones in propolis
from El Salvador.
Popova M, Bankova V, Spassov S, Tsvetkova I,
Naydenski C,
Silva MV, Tsartsarova M.
Institute of Organic Chemistry with Centre of Phytochemistry, Bulgarian Academy
of Sciences, Sofia.
2',3'-Dihydroxy-4,4'-dimethoxychalcone (1) and 2',3',4-trihydroxy-4'-methoxy-chalcone,
two new chalcones, were isolated from propolis from El Salvador. The compounds
showed significant antibacterial and antifungal activity and moderate toxicity
to Artemia salina nauplii.
PMID: 11531095 [PubMed - indexed for MEDLINE]
| 26: Z Naturforsch [C]. 2001 Jan-Feb;56(1-2):82-8. |
Egyptian propolis: 1-antimicrobial
activity and chemical composition of Upper Egypt propolis.
Hegazi AG, El Hady FK.
Department of Parasitology, National Research Center, Dokki, Giza, Egypt.
ahmedgaffer@mailer.suc.eun.eg
The antimicrobial activity of four propolis samples collected from Upper Egypt
against Staphylococcus aureus; Escherichia coli and Candida albicans was evaluated.
There was a variation in the antimicrobial activity according to the propolis
origin. Banisweif propolis showed the highest antimicrobial activity against
Staphylococcus aureus, Escherichia coli and Candida albicans, but Fayoum propolis
had moderate activity against all tested pathogens. Propolis collected from
Assiut and Souhag gave lower antimicrobial activity. Propolis samples were
investigated by GC/MS, 71 compounds were identified, 14 being new for propolis.
Banisweif propolis is characterized by the presence of 7 caffeate esters and
4 triterpenoids. Fayoum propolis showed the highest amount of lactic acid
and the presence of 3 chalcones. But Assiut propolis is characterized by the
presence of 4 prenylated coumarates. Souhag propolis is characterized by the
presence of 5 aliphatic dicarboxylic acids and some other new compounds to
propolis.
PMID: 11302219 [PubMed - indexed for MEDLINE]
| 27: J Chemother. 2000 Oct;12(5):390-5. |
Erratum in:
· J Chemother 2001 Feb;13(1):102.
In vitro antimicrobial activity
of propolis dry extract.
Drago L, Mombelli B, De Vecchi E,
Fassina MC, Tocalli L, Gismondo MR.
Clinical Microbiology, L. Sacco Teaching Hospital, University of Milan, Italy.
microbio@mailserver.unimi.it
In this study the antibacterial and antifungal properties of propolis, a natural
product of bees, have been investigated against different pathogens. Minimum
inhibitory concentrations (MICs) and minimum bactericidal concentrations (MBCs)
were determined according to NCCLS standards on 320 strains including Staphylococcus
aureus, Group A beta-hemolytic streptococci, Streptococcus pneumoniae, Moraxella
catarrhalis, Haemophilus influenzae, Klebsiella pneumoniae, Escherichia coli,
Proteus mirabilis, Pseudomonas aeruginosa and Candida albicans. Time-kill
curves were assessed for susceptible microorganisms, testing 0, 0.5, 1, 2,
4 x MIC for propolis, by counting viable bacteria after 0, 3, 6, 24 hours
and viable yeasts after 0, 3, 6, 24 and 48 hours. Propolis showed good antimicrobial
activity against most of the isolates, particularly S. pneumoniae, H. influenzae
and M. catarrhalis, but not against Enterobacteriaceae. Time-kill curves demonstrated
bacteriostatic rather than bactericidal activity of propolis, the latter being
evident only at high concentrations.
PMID: 11128558 [PubMed - indexed for MEDLINE]
| 28: Z Naturforsch [C]. 2000 Sep-Oct;55(9-10):785-9. |