PROPOLEOS Y CANCER
| Items 1 - 79 of 79 |
One page. |
| 1: Cancer Lett. 2006 Jan 10; [Epub ahead of print] |
Influence
of galangin on HL-60 cell proliferation and survival.
Bestwick
CS, Milne
L.
Molecular Nutrition Group, Gut Health Programme, Rowett Research Institute,
The effect of galangin, a flavonol component of
| 2: Life Sci. 2005 Dec 20; [Epub ahead of print] |
Therapeutic
effect of paclitaxel and propolis on lipid peroxidation and antioxidant system
in 7,12 dimethyl benz(a)anthracene-induced breast cancer in female Sprague
Dawley rats.
Padmavathi
R, Senthilnathan
P, Chodon
D, Sakthisekaran
D.
Department of Medical Biochemistry,
Breast cancer is one of the most common cancers in women of developed and
developing countries. The optimum management of which requires a multidisciplinary
approach including the use of certain biochemical and molecular markers. The
effect of propolis along with paclitaxel on 7,12 dimethyl benz(a)anthracene
(DMBA) induced experimental breast cancer was investigated in female Sprague
Dawley rats. Female Sprague Dawley rats were divided into five groups of six
animals each. Group I served as normal control animal. Group II animals received
DMBA (20 mg in 0.5 ml sunflower oil and 0.5 ml of saline) i.p. to develop
mammary tumor by the end of 90 days. Group III were breast cancer animals
treated with 33 mg paclitaxel/kg body weight (bw) weekly once for 4 weeks.
Group IV were breast cancer-bearing animals treated with 50 mg propolis/kg
bw for 30 days. Group V were breast cancer-bearing animals treated with both
paclitaxel and propolis as mentioned above. Administration of paclitaxel and
propolis effectively suppressed breast cancer, which is revealed by the decrease
in the extent of lipid peroxidation (LPO) with concomitant increase in the
activities of enzymic antioxidants (superoxide dismutase (SOD), catalase (CAT)
and glutathione peroxidase (GPx)) and non-enzymic antioxidants (reduced glutathione
(GSH), Vitamin C and Vitamin E) levels when compared to breast cancer-bearing
animals treated with either paclitaxel or propolis alone. From our results,
we conclude that propolis is a potent antioxidant and, when given in combination
with paclitaxel, offers maximum protection against DMBA induced mammary carcinogenesis.
| 3: Am J Kidney Dis. 2005 Dec;46(6):e125-9. |
Acute renal
failure induced by a Brazilian variety of propolis.
Li YJ, Lin
JL, Yang
CW, Yu CC.
Department of Nephrology,
Propolis is a resinous substance collected by honeybees and used in hive construction
and maintenance. Cumulative evidence suggests that propolis may have anti-inflammatory,
antibiotic, antioxidant, antihepatotoxic, and antitumor properties. In addition
to topical applications, products containing propolis have been used increasingly
as dietary supplements. Although reports of allergic reactions are not uncommon,
propolis is reputed to be relatively nontoxic. Its systemic toxicity is rarely
reported and hence may be underestimated. This is the first report of propolis-induced
acute renal failure. A 59-year-old man required hemodialysis for acute renal
failure. The patient had cholangiocarcinoma and had ingested propolis for
2 weeks before presentation. Renal function improved after propolis withdrawal,
deteriorated again after reexposure, and then returned to a normal level after
the second propolis withdrawal. This case indicates that propolis can induce
acute renal failure and emphasizes the need for vigilance and care when propolis
is used as a medicine or dietary supplement.
PMID: 16310564 [PubMed - indexed for MEDLINE]
| 4: Int J Radiat Oncol Biol Phys. 2005 Nov 15;63(4):1252-61. |
Caffeic acid
phenethyl ester preferentially sensitizes CT26 colorectal adenocarcinoma to
ionizing radiation without affecting bone marrow radioresponse.
Chen
YJ, Liao
HF, Tsai
TH, Wang
SY, Shiao
MS.
Department of Radiation Oncology,
PURPOSE: Caffeic acid phenethyl ester (
| 5: Br J Pharmacol. 2005 Dec;146(8):1139-47. |
Caffeic acid
phenethyl ester modulates Helicobacter pylori-induced nuclear factor-kappa
B and activator protein-1 expression in gastric epithelial cells.
Abdel-Latif
MM, Windle
HJ, Homasany
BS, Sabra
K, Kelleher
D.
Department of Clinical Medicine, Dublin Molecular Medicine Centre and Trinity
Centre for Health Sciences, St James's Hospital, Dublin 8, Ireland. abdellmm@tcd.ie
Caffeic acid phenethyl ester (CAPE), an active component of propolis from
honeybee hives (honeybee resin), has anti-inflammatory, anti-carcinogenic
and anti-bacterial properties. This study was designed to investigate the
anti-inflammatory effects of CAPE on Helicobacter pylori-induced NF-kappaB
and AP-
| 6: Cancer Lett. 2005 Oct 15; [Epub ahead of print] |
Dietary artepillin
C suppresses the formation of aberrant crypt foci induced by azoxymethane
in mouse colon.
Shimizu K, Das SK, Baba M, Matsuura Y, Kanazawa K.
Department of Life Science, Graduate
School of Science and Technology, Kobe University, Rokkodai, Nada-ku, Kobe
657-8501, Japan.
Artepillin C, a prenylated phenylpropanoid found specifically in Brazilian
propolis, has been shown to be a bioavailable antioxidant. In this study,
artepillin C was tested for colon cancer-preventing activity using azoxymethane-challenged
ddY mice. Oral doses of 80 and 160mg/kg body weight of propolis or 10mg/kg
of artepillin C (equi-amounts to 160mg propolis) reduced significantly the
frequency of colonic aberrant crypt foci (ACF) by 39.2, 43.7 and 43.4%, respectively.
In liver of the mice, glutathione S-transferase and NADPH:quinone reductase
activity increased with the doses of propolis or artepillin C, and an antioxidant-responsive
element (ARE) was found to be activated for binding DNA. Artepillin C is considered
to suppress the formation of colonic ACF through the activation of ARE and
induction of phase II enzymes in liver.
PMID: 16236434 [PubMed - as supplied by publisher]
| 7: Mol Carcinog. 2005 Dec;44(4):293-9. |
Artepillin
C in Brazilian propolis induces G(0)/G(1) arrest via stimulation of Cip1/p21
expression in human colon cancer cells.
Shimizu K, Das SK, Hashimoto T,
Sowa Y, Yoshida T, Sakai T, Matsuura Y, Kanazawa K.
Department of Life Science, Graduate
School of Science and Technology, Kobe University, Kobe, Japan.
Potential chemopreventive agents exist in foods. Artepillin C in Brazilian
propolis was investigated for its effects on colon carcinogenesis. We had
found that artepillin C was a bioavailable antioxidant, which could be incorporated
into intestinal Caco-2 and hepatic HepG2 cells without any conjugation and
inhibited the oxidation of intracellular DNA. Artepillin C was then added
to human colon cancer WiDr cells. It dose-dependently inhibited cell growth,
inducing G(0)/G(1) arrest. The events involved a decrease in the kinase activity
of a complex of cyclin D/cyclin-dependent kinase 4 and in the levels of retinoblastoma
protein phosphorylated at Ser 780 and 807/811. The inhibitors of the complex,
Cip1/p21 and Kip1/p27, increased at the protein level. On the other hand,
Northern blotting showed that artepillin C did not affect the expression of
Kip1/p27 mRNA. According to the experiments using isogenic human colorectal
carcinoma cell lines, artepillin C failed to induce G(0)/G(1) arrest in the
Cip1/p21-deleted HCT116 cells, but not in the wild-type HCT116 cells. Artepillin
C appears to prevent colon cancer through the induction of cell-cycle arrest
by stimulating the expression of Cip1/p21 and to be a useful chemopreventing
factor in colon carcinogenesis.
PMID: 16224795 [PubMed - indexed for MEDLINE]
| 8: J Nutr Biochem. 2005 Oct 5; [Epub ahead of print] |
Inhibitory
effects of caffeic acid phenethyl ester on cancer cell metastasis mediated
by the down-regulation of matrix metalloproteinase expression in human HT1080
fibrosarcoma cells.
Hwang
HJ, Park
HJ, Chung
HJ, Min
HY, Park
EJ, Hong
JY, Lee
SK.
Department of Pharmacy, College of Pharmacy, Ewha Woman's University, Seodaemun-Ku,
Seoul 120-750, South Korea.
Caffeic acid phenethyl ester (CAPE) derived from honeybee propolis has been
used as a folk medicine. Recent study also revealed that CAPE has several
biological activities including antioxidation, anti-inflammation and inhibition
of tumor growth. The present study investigated the effect of CAPE on tumor
invasion and metastasis by determining the regulation of matrix metalloproteinases
(MMPs). Matrix metalloproteinases, which are zinc-dependent proteolytic enzymes,
play a pivotal role in tumor metastasis by cleavage of extracellular matrix
(ECM) as well as nonmatrix substrates. On this line, we examined the influence
of CAPE on the gene expression of MMPs (MMP-2, MMP-9, MT1-MMP), tissue inhibitor
of metalloproteinase-2 (TIMP-2) and in vitro invasiveness of human fibrosarcoma
cells. Dose-dependent decreases in MMP and TIMP-2 mRNA levels were observed
in CAPE-treated HT1080 human fibrosarcoma cells as detected by reverse transcriptase-polymerase
chain reaction (RT-PCR). Gelatin zymography analysis also exhibited a significant
down-regulation of MMP-2 and MMP-9 expression in HT1080 cells treated with
CAPE compared to controls. In addition, CAPE inhibited the activated MMP-2
activity as well as invasion, motility, cell migration and colony formation
of tumor cells. These data therefore provide direct evidence for the role
of CAPE as a potent antimetastatic agent, which can markedly inhibit the metastatic
and invasive capacity of malignant cells.
PMID: 16214327 [PubMed - as supplied by publisher]
| 9: Biol Pharm Bull. 2005 Oct;28(10):1928-33. |
Effects of
local administration of propolis and its polyphenolic compounds on tumor formation
and growth.
Orsolic
N, Terzic
S, Mihaljevic
Z, Sver
L, Basic
I.
Department of Animal Physiology, Faculty of Science, University of Zagreb;
10000 Zagreb, Rooseveltov trg 6, Croatia. norsolic@yahoo.com
Many dietary constituents are chemopreventive in animal models, and experiments
with cultured cells are revealing various potential mechanisms of action.
Compounds classified as blocking agents can prevent, or greatly reduce, initiation
of carcinogenesis, or suppressing agents can act on cell proliferation. Caffeic
acid (CA) and caffeic acid phenethyl ester (CAPE), members of the polyphenolic
compounds, are present in high concentrations in medicinal plants and propolis,
a natural beehive product. A water-soluble extract of propolis (WSDP) and
two components of propolis, CA and CAPE were investigated for direct antitumor
activity in vivo and in vitro. The local presence of CA and CAPE in the tissue
caused a significant delay in tumor formation and increased life span 29.30
to 51.73%, respectively. CA and CAPE, but not WSDP, significantly suppressed
human HeLa cervical carcinoma cell proliferation in vitro. Based on these
results, we postulate that the antitumor activity of polyphenolic compounds
includes direct cytotoxic effects on tumor cells.
PMID: 16204948 [PubMed - indexed for MEDLINE]
| 10: Biomed Pharmacother. 2005 Dec;59(10):561-70. Epub 2005 Aug 10. |
Antitumor,
hematostimulative and radioprotective action of water-soluble derivative of
propolis (WSDP).
Orsolic
N, Basic
I.
Department of Animal Physiology, Faculty of Science, University of Zagreb,
Rooseveltov trg 6, Croatia. norsolic@yahoo.com
Several studies suggest that dietary supplementation with antioxidant can
influence the response to chemotherapy as well as the development of adverse
side effects caused by treatment with chemotherapeutic agents. Using CBA mouse
model, we investigated a clinically potential use of a water-soluble derivative
of propolis (WSDP) in the treatment of various cytopenias induced by radiation
and/or chemotherapy. Also, the antimetastatic efficiency of WSDP given intraperitoneally
alone or in combination with chemotherapeutic agents and their effects on
the blood leukocytes count as well as on hematopoiesis were studied. Tumor
was a transplantable mammary carcinoma (MCa) of CBA mouse. Metastases in the
lung were generated by injecting viable tumor cells intravenously (iv). WSDP
(50 or 150 mg/kg) exerted a significant antimetastatic effect (P < 0.001)
when given either before or after tumor cell inoculation. In combined treatment
WSDP and Epirubicin profoundly inhibited metastasis formation; this synergistic
effect is maximal when Epirubicin and WSDP were administrated after tumor
cell inoculation. Positive outcome of combined treatment with WSDP and Epirubicin
was also found regarding the number of red and white blood cells in peripheral
blood while in mice treated with Epirubicin alone the significant drop in
all hematological parameters was noticed on day 13 after tumor cell inoculation.
Furthermore, when WSDP (50 mg/kg) was given perorally (po) for 20 consecutive
days an increased number of exogenous CFUs was found in treated mice. WSDP
given either for 20 or 40 days increased cellularity of hematopoietic tissue
and the number of leucocytes in peripheral blood; prolonged treatment with
WSDP also elevated myeloid and megakaryocytic types of CFUs. To conclude,
these findings indicate that the combination of WSDP with chemotherapeutics
could increase the antimetastatic potential of chemotherapeutic agents; these
findings suggest the benefits of potential clinical trials using WSDP combined
with chemotherapeutic agents in order to maximize their antitumor activity
and minimize postchemotherapeutic or radiotherapeutic deteriorated reactions.
PMID: 16202559 [PubMed - indexed for MEDLINE]
| 11: Leuk Res. 2005 Nov;29(11):1343-6. |
Evaluation
of Manisa propolis effect on leukemia cell line by telomerase activity.
Gunduz C, Biray C, Kosova B, Yilmaz B, Eroglu Z, Sahin F, Omay SB, Cogulu O.
Ege University, Faculty of Medicine,
Department of Medical Biology, Izmir, Turkey.
Propolis is a resinous substance which is used by bees to repair and maintain
their hives. It has more than 180 compounds including flavonoids, phenolic
acids and its esters which have anti-inflammatory, antibacterial, antiviral,
immunomodulatory, antioxidant and antiproliferative effects. Propolis is shown
to inhibit cell division and protein synthesis. However the exact mechanism
underlying antitumor effect is not clearly described. On the other hand progressive
telomere shortening to a critical level results with senescence of normal
cells by inducing apoptosis and telomerase prevents erosion of telomeres.
In this study we aimed to evaluate hTERT ratios in propolis-treated T-cell
acute lymphoblastic leukemia (CCFR-CEM) cell line. Cell counts and cell viability
of propolis-treated and propolis-free T-cell acute lymphoblastic leukemia
(CCFR-CEM) cell line were assessed by trypan blue dye exclusion test and MTT
assay. The LightCycler instrument was used (online real-time PCR) for the
quantification of hTERT in CCFR-CEM cell line. The hTERT ratio significantly
decreased 60 and 93% after 24 and 72 h respectively compared to the initial
value of the cells incubated with propolis. It had almost no cytotoxic effect
and caused 30, 30, 22 and 12% decrease in cell counts after 24, 48, 72 and
96 h respectively which is statistically significant. In conclusion propolis
may show antitumor and apoptotic effect via inhibiting telomerase expression
besides the mechanisms which have been described previously.
PMID: 16055186 [PubMed - indexed for MEDLINE]
| 12: Int Immunopharmacol. 2005 Oct;5(11):1652-7. |
Effects of
Turkish pollen and propolis extracts on respiratory burst for K-562 cell lines.
Aliyazicioglu Y,
Deger O, Ovali E, Barlak Y, Hosver I, Tekelioglu Y,
Karahan SC.
Department of Biochemistry, Faculty
of Medicine, Ondokuz Mayis University, Samsun, 55139, Turkey.
Bee-collected pollen and propolis are apicultural products which are composed
of nutritionally valuable substances and contain considerable amounts of polyphenol
substances which may act as potent antioxidants. We wanted to show if respiratory
burst within a cancer cell lines could be influenced when incubated with pollen
and propolis extracts or not. Pollen and propolis extracts at concentrations
of 50, 25, 12.5 and 0 mg/ml were prepared by dimethyl sulfoxide (DMSO). K-562
cell cultures and mononuclear cell (MNC) cultures prepared from a peripheral
blood sample to serve as control cells were incubated with extracts for 24
h. Determination of respiratory burst was carried out by intracellular dichlorofluorescein
(DCFH) test by using flow-cytometric fluorescence analysis. While about 90%
and 66% fluorescence was detected at zero concentrations for both K-562 and
MNC cultures, fluorescence positivity decreased (between 3.8% and 11.8%) as
concentrations of both propolis and pollen extracts increased for K-562 cell
culture, but unchanged (between 20% and 83%) for MNC culture. It was concluded
that pollen and propolis extracts inhibit respiratory burst within cancer
cell lines probably by their antioxidant potentials.
PMID: 16039555 [PubMed - indexed for MEDLINE]
| 13: Clin Chim Acta. 2005 Dec;362(1-2):57-64. Epub 2005 Jul 6. |
Caffeic acid
phenyl ester in propolis is a strong inhibitor of matrix metalloproteinase-9
and invasion inhibitor: isolation and identification.
Jin UH, Chung TW, Kang SK, Suh SJ, Kim JK, Chung KH, Gu YH, Suzuki I, Kim CH.
Department of Biochemistry and
Molecular Biology, Dongguk University College of Oriental Medicine and National
Research Laboratory for Glycobiology, Kyungbuk, Korea.
BACKGROUND: Propolis has been used as a folk medicine and has several proven
biological activities. Herbal remedies recommended for cancer therapies in
Korea. METHODS: Matrix metalloproteinase (MMP)-9-inhibitory activity of propolis
has been assessed. CAPE as an acting compound was isolated and molecular structure
was determined. Anti-invasion activity of CAPE was assayed using hepatocarcinoma
cells. RESULTS: Propolis ethanol extracts showed a strong inhibitory effect
of MMP-9 activity, which is known to be involved in tumor cell invasion and
metastasis in a concentration-dependent manner on zymography. Assay guided
fractionation led to the isolation of a caffeic acid phenyl ester (CAPE) as
the compound responsible for the anti-MMP-9 activity. CAPE was obtained by
reversed-phase HPLC, and its structure was elucidated by fast atom bombardment
mass spectrometry and tandem mass spectrometry. The purified CAPE inhibited
MMP-9 activity with the IC(50) of 1.0-2.0 nmol/l. CONCLUSIONS: CAPE possesses
selective antiproliferative activity toward hepatocaricoma cell line Hep3B,
but not primary cultured mouse hepatocytes.
PMID: 16004979 [PubMed - indexed for MEDLINE]
| 14: Biol Pharm Bull. 2005 Jun;28(6):1025-30. |
Two related
cinnamic Acid derivatives from Brazilian honey bee propolis, baccharin and
drupanin, induce growth inhibition in allografted sarcoma s-
Mishima S, Ono Y, Araki Y, Akao Y, Nozawa Y.
API Co. Ltd., R&D, Gifu,
Japan.
Honey bee propolis is rich in cinnamic acid derivatives. Baccharin and drupanin
from Brazilian honey bee propolis are cinnamic acid derivatives that contain
prenyl moieties. We previously isolated these two compounds and demonstrated
that they induce an apoptotic event in several tumor cell lines. In this study,
we examined the tumoricidal activity of baccharin and drupanin in mice allografted
with sarcoma S-180 and also studied the genotoxic effects on normal splenocytes
using the alkaline single cell gel (comet) assay. We found that both baccharin
and drupanin effectively suppressed growth of the tumor. Furthermore, these
compounds induced a significant genotoxic effect on the tumor cells in comparison
with normal splenocytes. Thus, baccharin and drupanin are potent tumor suppressive
components of honeybee propolis.
PMID: 15930739 [PubMed - indexed for MEDLINE]
| 15: Biochem Pharmacol. 2005 Jun 15;69(12):1815-27. |
Chrysin induces
G1 phase cell cycle arrest in C6 glioma cells through inducing p21Waf1/Cip1
expression: involvement of p38 mitogen-activated protein kinase.
Weng
MS, Ho YS, Lin
JK.
Graduate Institute of Biochemistry and Molecular Biology, College of Medicine,
National Taiwan University, No. 1, Section 1, Jen-Ai Road, Taipei 10018, Taiwan.
Flavonoids are a broadly distributed class of plant pigments, universally
present in plants. They are strong anti-oxidants that can inhibit carcinogenesis
in rodents. Chrysin (5,7-dihydroxyflavone) is a natural and biologically active
compound extracted from many plants, honey, and propolis. It possesses potent
anti-inflammatory, anti-oxidant properties, promotes cell death, and perturbing
cell cycle progression. However, the mechanism by which chrysin inhibits cancer
cell growth remains poorly understood. Therefore, we developed an interest
in the relationship between MAPK signaling pathways and cell growth inhibition
after chrysin treatment in rat C6 glioma cells. Cell viability assay and flow
cytometric analysis suggested that chrysin exhibited a dose-dependent and
time-dependent ability to block rat C6 glioma cell line cell cycle progression
at the G1 phase. Western blotting analysis showed that the levels of Rb phosphorylation
in C6 glioma cells exposed to 30 microM chrysin for 24h decreased significantly.
We demonstrated the expression of cyclin-dependent kinase inhibitor, p21(Waf1/Cip1),
to be significantly increased, but the p53 protein level did not change in
chrysin-treated cells. Both cyclin-dependent kinase 2 (CDK2) and 4 (CDK4)
kinase activities were reduced by chrysin in a dose-dependent manner. Furthermore,
chrysin also inhibited proteasome activity. We further showed that chrysin
induced p38-MAPK activation, and using a specific p38-MAPK inhibitor, SB203580,
attenuated chrysin-induced p21(Waf1/Cip1) expression. These results suggest
that chrysin exerts its growth-inhibitory effects either through activating
p38-MAPK leading to the accumulation of p21(Waf1/Cip1) protein or mediating
the inhibition of proteasome activity.
PMID: 15869744 [PubMed - indexed for MEDLINE]
| 16: J Dent Res. 2005 May;84(5):468-73. |
Properties
of BK(Ca) channels in oral keratinocytes.
Shieh DB, Yang SR, Shi XY, Wu YN, Wu SN.
Institute of Oral Medicine, National
Cheng Kung University Medical College, No. 1, University Road, Tainan 701,
Taiwan.
Keratinocytes are important for epithelial antimicrobial barrier function.
The activity of ion channels can affect the proliferation of keratinocytes.
Little is known about Ca2+-activated K+ currents in these cells. Ion currents
in normal human oral keratinocytes were characterized with a patch-clamp technique.
In whole-cell configuration, depolarizing pulses evoked K+ outward currents
(I(K)) in oral keratinocytes. Iberiotoxin (200 nM) and paxilline (1 microM)
suppressed I(K); however, neither apamin (200 nM) nor 5-hydroxydecanoate (30
microM) had any effects on it. Caffeic acid phenethyl ester, a compound of
honeybee propolis, increased I(K) with an EC50 value of 12.8 +/- 1.2 microM.
In inside-out patches, a BK(Ca) channel was observed in keratinocytes, but
not in oral squamous carcinoma (OCE-M1) cells. Caffeic acid phenethyl ester
or cinnamyl-3,4-dihydroxy-alpha-cyanocinnamate applied to the intracellular
surface of a detached patch increased BK(Ca)-channel activity. The results
demonstrate that the properties of BK(Ca) channels in normal human oral keratinocytes
are similar to those described in other types of cells. Caffeic acid derivatives
can also stimulate BK(Ca)-channel activity directly.
PMID: 15840785 [PubMed - indexed for MEDLINE]
| 17: Biol Pharm Bull. 2005 Apr;28(4):694-700. |
Synergistic
antitumor effect of polyphenolic components of water soluble derivative of
propolis against Ehrlich ascites tumour.
Orsolic
N, Kosalec
I, Basic
I.
Department of Animal Physiology, Faculty of Science, University of Zagreb,
Croatia.
Effect of two preparation (Croatian and Brazilian) of water-soluble derivative
of propolis (WSDP), caffeic acid, quercetin, chrysin, naringenin (components
present in WSDP) on the development of Ehrlich ascites tumour (EAT) was evaluated.
Test components (50 mg/kg) were given perorally or intraperitoneally 2 h prior
the intraperitonel injection of EAT (2 x 10(6)) cells. It was observed that
all test compounds effectively inhibited tumour growth and the proliferation
of EAT. The volume of ascitic fluid induced by EAT cells and total number
of cells present in the peritoneal cavity was markedly reduced in EAT-bearing
mice treated with test components. In treated mice the number of polymorphonuclear
(PMN) cells in the peritoneal cavity was increased while the number of macrophages
was decreased. The macrophage spreading activity revealed that WSDP and all
test compounds affected the functional state of macrophages increasing their
tumorcidal activity; the effect of WSDP was most pronounced indicating synergistic
effect of components present in WSDP. Antitumor activity of WSDP may be the
result of different specific mechanism(s) of flavonoids present as compared
to individual flavonoid given alone. It is likely that the part of antitumor
efficacy of test components against EAT cells was the results of increased
activity of macrophages.
PMID: 15802812 [PubMed - indexed for MEDLINE]
| 18: Nat Prod Res. 2005 Feb;19(2):183-8. |
Natural product
propolis: chemical composition.
Sahinler
N, Kaftanoglu
O.
Mustafa Kemal University, Faculty of Agriculture, Department of Animal Science,
Hatay, Turkey. nsahinler@mku.edu.tr
The chemical composition of propolis from East Mediterranean (Hatay, Adana
and Mersin) was studied in order to determine the major compounds by using
GC-MS. In this study, the ethanolic extract of propolis prepared by mixing
1900mL 70% ethanol and 100g propolis was used. Chemical analysis of propolis
extracts indicated that the propolis samples had high concentrations of the
aromatic acids, esters and other derivatives which are responsible for the
anti-bacterial, anti-fungal, anti-viral, anti-inflammatory and anti-cancer
properties of propolis such as benzyl cinnamate, methyl cinnamate, caffeic
acid, cinnamyl cinnamate and cinnamoylglcine besides the most common compounds
as fatty acid, terpenoids, esters, alcohols hydrocarbons and aromatic acids.
PMID: 15715264 [PubMed - indexed for MEDLINE]
| 19: FEBS Lett. 2005 Jan 31;579(3):705-11. |
Chrysin suppresses
lipopolysaccharide-induced cyclooxygenase-2 expression through the inhibition
of nuclear factor for IL-6 (NF-IL6) DNA-binding activity.
Woo KJ, Jeong YJ, Inoue H, Park JW, Kwon TK.
Department of Immunology, School
of Medicine, Keimyung University, 194 DongSan-Dong Jung-Gu, Taegu 700-712,
South Korea.
Chrysin is a natural, biologically active compound extracted from many plants,
honey and propolis. It possesses potent anti-inflammation, anti-cancer and
anti-oxidation properties. The mechanism by which chrysin suppresses COX-2
expression remains poorly understood. In the present report, we investigated
the effect of chrysin on the expression of COX-
| 20: Ann N Y Acad Sci. 2004 Dec;1030:501-7. |
Inhibition
of cyclooxygenase-2 expression and restoration of gap junction intercellular
communication in H-ras-transformed rat liver epithelial cells by caffeic acid
phenethyl ester.
Lee KW, Chun KS, Lee JS, Kang KS, Surh YJ, Lee HJ.
Department of Food Science and
Technology, School of Agricultural Biotechnology, Seoul National University,
Seoul 151-742, South Korea.
One of the most frequent defects in human cancers is the uncontrolled activation
of the ras signaling pathways. Increased expression of cyclooxygenase-2 (COX-2)
and inhibition of gap junction intercellular communication (GJIC) have been
frequently observed in several forms of human malignancies. The present study
investigated the effects of caffeic acid phenethyl ester (CAPE), a chemopreventive
phytochemical derived from honey propolis, on COX-2 expression and GJIC in
Harvey-ras-transformed WB-F344 rat liver epithelial cells (H-ras WB cells).
H-ras induced COX-2 expression in WB-F344 rat liver epithelial cells (WB cells).
H-ras WB cells also exhibited complete inhibition of GJIC and predominant
unphosphorylation of connexin 43 (Cx43), a major protein modulating GJIC.
CAPE significantly inhibited the constitutive expression of COX-2 and restored
the disrupted GJIC through the phosphorylation of Cx43 at a concentration
of 12.5 microM in H-ras WB cells. Although the molecular basis for the cancer
chemopreventive activity of CAPE is not completely understood, several studies
suggest that CAPE is a potent and specific inhibitor of the transcription
factor nuclear factor kappaB (NF-kappaB) activation. We also found that CAPE
significantly inhibited H-ras-induced NF-kappaB DNA-binding activity without
affecting the activation of extracellular signal-regulated kinase and p38
mitogen-activated protein kinase, which are major intracellular molecules
involved in the Ras signaling pathways. In conclusion, CAPE may exert cancer
chemopreventive effects through the inhibition of COX-2 expression and the
restoration of disrupted GJIC induced by H-ras, possibly by targeting NF-kappaB.
PMID: 15659835 [PubMed - indexed for MEDLINE]
| 21: Environ Mol Mutagen. 2005;45(1):8-16. |
Modifying
effect of propolis on dimethylhydrazine-induced DNA damage but not colonic
aberrant crypt foci in rats.
de Lima RO, Bazo AP, Said RA, Sforcin JM, Bankova V, Darros BR, Salvadori DM.
Departamento de Patologia, Faculdade de Medicina, Universidade Estadual Paulista,
Botucatu, Sao Paulo, Brazil.
Propolis is a honeybee product
with several biological and therapeutic properties, including antimutagenic
and anticarcinogenic activities. The effects of an aqueous extract of propolis
(AEP) were evaluated on the formation of 1,2-dimethylhydrazine (DMH)-induced
aberrant crypt foci (ACF) and DNA damage in the colon of male Wistar rats
by the ACF and Comet assays, respectively. AEP was administered orally at
0.01%, 0.03%, 0.1%, and 0.3% in the drinking water, which resulted in doses
of approximately 12, 34, 108, and 336 mg/kg body weight/day. Animals were
also given a single subcutaneous injection of 40 mg/kg DMH and sacrificed
4 hr later for evaluating DNA damage, or 4 doses of 40 mg/kg DMH, administered
2 doses/week for 2 weeks, and sacrificed 12 weeks after the last injection
for evaluating ACF development in the distal colon. Administration of AEP
either simultaneously with or after the DMH treatment resulted in no statistically
significant reduction of ACF. In contrast, 0.01%, 0.03%, and 0.3% AEP, given
simultaneously with DMH, reduced DNA damage induction in the mid and distal
colon. However, 0.3% AEP alone increased DNA damage in the colon. In conclusion,
AEP had no effect on the formation of DMH-induced ACF in rat colon, but it
modulated DMH-induced DNA damage in colon cells. Further investigations are
recommended in order to establish the conditions under which propolis produces
either protective or deleterious effects. 2004
Wiley-Liss, Inc.
PMID: 15605358 [PubMed - indexed for MEDLINE]
| 22: Life Sci. 2004 Dec 17;76(5):545-58. |
Chilean propolis:
antioxidant activity and antiproliferative action in human tumor cell lines.
Russo A, Cardile V, Sanchez F, Troncoso N, Vanella A, Garbarino JA.
Department of Biological Chemistry,
Medical Chemistry and Molecular Biology, University of Catania, v.le A. Doria
6, 95125 Catania-Italy. alrusso@unict.it
Propolis, a natural product derived from plant resins collected by honeybees,
has been used for thousands of years in traditional medicine all over the
world. The composition of the propolis depends upon the vegetation of the
area from where it was collected and on the bee species. In this study, we
investigated the antioxidant activity of a propolis sample, provided by NATURANDES-CHILE,
collected in a temperate region of central Chile. In addition, this natural
compound was tested for its antiproliferative capacity on KB (human mouth
epidermoid carcinoma cells), Caco-2 (colon adenocarcinoma cells) and DU-145
(androgen-insensitive prostate cancer cells) human tumor cell lines. Results
showed that this Chilean propolis sample exhibits interesting biological properties,
correlated with its chemical composition and expressed by its capacity to
scavenge free radicals and to inhibit tumor cell growth.
PMID: 15556167 [PubMed - indexed for MEDLINE]
| 23: Biochem Biophys Res Commun. 2004 Dec 24;325(4):1215-22. |
Chrysin-induced
apoptosis is mediated through caspase activation and Akt inactivation in U937
leukemia cells.
Woo
KJ, Jeong
YJ, Park
JW, Kwon
TK.
Department of Immunology, School of Medicine, Keimyung University, 194 DongSan-Dong
Jung-Gu, Taegu 700-712, Republic of Korea. kwontk@dsmc.or.kr
Chrysin is a natural, biologically active compound extracted from many plants,
honey, and propolis. It possesses potent anti-inflammation, anti-cancer, and
anti-oxidation properties. The mechanism by which chrysin initiates apoptosis
remains poorly understood. In the present report, we investigated the effect
of chrysin on the apoptotic pathway in U937 human promonocytic cells. We show
that chrysin induces apoptosis in association with the activation of caspase
3 and that Akt signal pathway plays a crucial role in chrysin-induced apoptosis
in U937 cells. Furthermore, we have shown that inhibition of Akt phosphorylation
in U937 cells by the specific PI3K inhibitor, LY294002 significantly, enhanced
apoptosis. Overexpression of a constitutively active Akt (myr-Akt) in U937
cells inhibited the induction of apoptosis, activation of caspase 3, and PLC-gamma1
cleavage by chrysin. Together, these findings suggest that the Akt pathway
plays a major role in regulating the apoptotic response of human leukemia
cells to chrysin and raise the possibility that combined interruption of chrysin
and PI3K/Akt-related pathways may represent a novel therapeutic strategy in
hematological malignancies.
PMID: 15555556 [PubMed - indexed for MEDLINE]
| 24: Oncol Res. 2004;14(9):415-26. |
Resveratrol and
propolis as necrosis or apoptosis inducers in human prostate carcinoma cells.
Scifo
C, Cardile
V, Russo
A, Consoli
R, Vancheri
C, Capasso
F, Vanella
A,