BIBLIOGRAFIA PROPOLEOS ANTIBIOTICO

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1: Rev Bras Enferm. 2005 Mar-Apr;58(2):208-13.

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[Wound treatment: scientific production analysis published in the Revista Brasileira de Enfermagem from 1970-2003]

[Article in Portuguese]

Pereira AL, Bachion MM.

Aluna do Programa de Pos-Graduacao Mestrado em Enfermagem, da Faculdade de Enfermagem da Universidade Federal de Goias. ginha.ange@bol.com.br

This systematic review aimed to analyze the scientific production concerning the use of products in the treatment of wounds, published by REBEn from 1970 to 2003. We consulted all numbers of the journal published in the period, identifying 41 articles of interest. After an appraisal reading we included 11 papers in the sample. We identified a larger publication in the decade of 1990, prevailing authors of the attendance area, almost-experimental plan and experience repor. The studied products were papain, propolis, sugar, Unna boot, activated coal, transparent film, amniotic membrane, albumin, collagenase, hydrocolloid dressing, calcium alginate, anti-bacterial agents, essential greasing acids, viscose fibers and polyester. The findings demonstrated they denote positive effects of the use of the products.

PMID: 16334190 [PubMed - in process]


2: Int J Antimicrob Agents. 2005 Nov;26(5):343-56.

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Antimicrobial activity of flavonoids.

Cushnie TP, Lamb AJ.

Flavonoids are ubiquitous in photosynthesising cells and are commonly found in fruit, vegetables, nuts, seeds, stems, flowers, tea, wine, propolis and honey. For centuries, preparations containing these compounds as the principal physiologically active constituents have been used to treat human diseases. Increasingly, this class of natural products is becoming the subject of anti-infective research, and many groups have isolated and identified the structures of flavonoids possessing antifungal, antiviral and antibacterial activity. Moreover, several groups have demonstrated synergy between active flavonoids as well as between flavonoids and existing chemotherapeutics. Reports of activity in the field of antibacterial flavonoid research are widely conflicting, probably owing to inter- and intra-assay variation in susceptibility testing. However, several high-quality investigations have examined the relationship between flavonoid structure and antibacterial activity and these are in close agreement. In addition, numerous research groups have sought to elucidate the antibacterial mechanisms of action of selected flavonoids. The activity of quercetin, for example, has been at least partially attributed to inhibition of DNA gyrase. It has also been proposed that sophoraflavone G and (-)-epigallocatechin gallate inhibit cytoplasmic membrane function, and that licochalcones A and C inhibit energy metabolism. Other flavonoids whose mechanisms of action have been investigated include robinetin, myricetin, apigenin, rutin, galangin, 2,4,2'-trihydroxy-5'-methylchalcone and lonchocarpol A. These compounds represent novel leads, and future studies may allow the development of a pharmacologically acceptable antimicrobial agent or class of agents.

Publication Types:

·       Review


PMID: 16323269 [PubMed - indexed for MEDLINE]


3: Am J Kidney Dis. 2005 Dec;46(6):e125-9.

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Acute renal failure induced by a Brazilian variety of propolis.

Li YJ, Lin JL, Yang CW, Yu CC.

Department of Nephrology, Chang Gung Memorial Hospital, Taipei, Taiwan.

Propolis is a resinous substance collected by honeybees and used in hive construction and maintenance. Cumulative evidence suggests that propolis may have anti-inflammatory, antibiotic, antioxidant, antihepatotoxic, and antitumor properties. In addition to topical applications, products containing propolis have been used increasingly as dietary supplements. Although reports of allergic reactions are not uncommon, propolis is reputed to be relatively nontoxic. Its systemic toxicity is rarely reported and hence may be underestimated. This is the first report of propolis-induced acute renal failure. A 59-year-old man required hemodialysis for acute renal failure. The patient had cholangiocarcinoma and had ingested propolis for 2 weeks before presentation. Renal function improved after propolis withdrawal, deteriorated again after reexposure, and then returned to a normal level after the second propolis withdrawal. This case indicates that propolis can induce acute renal failure and emphasizes the need for vigilance and care when propolis is used as a medicine or dietary supplement.

Publication Types:

·       Case Reports


PMID: 16310564 [PubMed - indexed for MEDLINE]


4: J Agric Food Chem. 2005 Nov 16;53(23):8957-62.

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Evaluation of the cytotoxicity, genotoxicity, mutagenicity, and antimutagenicity of propolis from Tucuman, Argentina.

Nieva Moreno MI, Zampini IC, Ordonez RM, Jaime GS, Vattuone MA, Isla MI.

Instituto de Estudios Vegetales Dr Antonio Rodolfo Sampietro, Facultad de Bioquimica, Quimica y Farmacia, Universidad Nacional de Tucuman, Ayacucho 461, 4000 San Miguel de Tucuman, Argentina.

This study evaluates the toxic, genotoxic/mutagenic, and antimutagenic effects of propolis extract from Amaicha del Valle, Tucuman, Argentina. The cytotoxicity assays carried out with the lethality test of Artemia salina revealed that the LD50 was around 100 microg/mL. Propolis extracts showed no toxicity to Salmonella typhimurium TA98 and TA100 strains and Allium cepa at concentrations that have antibiotic and antioxidant activities. Otherwise, for the testing doses, neither genotoxicity nor mutagenicity was found in any sample. The propolis extracts were able to inhibit the mutagenesis of isoquinoline (IQ) and 4-nitro o-phenylenediamine (NPD) with ID50 values of 40 and 20 microg/plate, respectively. From this result, the studied propolis may be inferred to contain some chemical compounds capable of inhibiting the mutagenicity of direct-acting and indirect-acting mutagens. A compound isolated from Amaicha del Valle propolis, 2',4'-dihydroxychalcone, showed cytotoxic activity (LC50 values of 0.5 microg/mL) but was not genotoxic or mutagenic. Furthermore, this compound was able to inhibit the mutagenicity of IQ (ID50 values of 1 microg/plate) but was unable to inhibit the mutagenicity of NPD. Our results suggest a potential anticarcinogenic activity of Amaicha del Valle propolis and the chalcone isolated from it.

PMID: 16277388 [PubMed - indexed for MEDLINE]


5: Mem Inst Oswaldo Cruz. 2005 Aug;100(5):563-6. Epub 2005 Sep 15.

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Propolis: anti-Staphylococcus aureus activity and synergism with antimicrobial drugs.

Fernandes Junior A, Balestrin EC, Betoni JE, Orsi Rde O, da Cunha Mde L, Montelli AC.

Departamento de Microbiologia e Imunologia, Instituto de Biociencias, Universidade Estadual Paulista Julio de Mesquita Filho, Unesp, Botucatu, SP, Brazil. ary@ibb.unesp.br

Propolis is a natural resinous substance collected by bees from tree exudates and secretions.
Its antimicrobial activity has been investigated and inhibitory action on Staphylococcus aureus growth was evaluated. The in vitro synergism between ethanolic extract of propolis (EEP) and antimicrobial drugs by two susceptibility tests (Kirby and Bauer and E-Test) on 25 S. aureus strains was evaluated. Petri dishes with sub-inhibitory concentrations of EEP were incubated with 13 drugs using Kirby and Bauer method and synergism between EEP and five drugs [choramphenicol (CLO), gentamicin (GEN), netilmicin (NET), tetracycline (TET), and vancomycin (VAN)] was observed. Nine drugs were assayed by the E-test method and five of them exhibited a synergism [CLO, GEN, NET, TET, and clindamycin (CLI)]. The results demonstrated the synergism between EEP and antimicrobial drugs, especially those agents that interfere on bacterial protein synthesis.

PMID: 16184236 [PubMed - indexed for MEDLINE]


6: J Ethnopharmacol. 2005 Oct 3;101(1-3):243-8.

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Detection of galangin-induced cytoplasmic membrane damage in Staphylococcus aureus by measuring potassium loss.

Cushnie TP, Lamb AJ.

School of Pharmacy, The Robert Gordon University, Schoolhill, Aberdeen, Scotland AB10 1FR., UK.

Galangin is one of the active antimicrobial principles of propolis or 'bee glue' and Helichrysum aureonitens, a perennial herb used by South African indigenes to treat infection. The effect of this compound and antibacterial agents with known mechanisms of action upon the cytoplasmic membrane integrity of Staphylococcus aureus was investigated by comparing potassium loss profiles from bacterial cell suspensions. Using an agar dilution assay, the minimum inhibitory concentrations (MICs) of the flavonol galangin, the bacteriostatic antibiotic novobiocin and the bactericidal antibiotic penicillin G against Staphylococcus aureus NCTC 6571 were determined as being 50 microg/mL, 62.5 ng/mL and 31.3 ng/mL, respectively. When 5x10(7)cfu/mL Staphylococcus aureus were suspended in 'potassium-free' media containing 50 microg/mL galangin, a 60-fold decrease in viability was observed after 12 h. Populations of 1x10(9) cfu/mL Staphylococcus aureus incubated for 12 h in 50 microg/mL galangin lost 21% more potassium than untreated control populations. Novobiocin had no effect on potassium loss, but populations incubated in 31.3 ng/mL penicillin G exhibited a 6% increase in potassium loss. This data clearly demonstrates that galangin causes a significant increase in potassium loss from Staphylococcus aureus cells, which may be attributed to either direct damage to the cytoplasmic membrane or indirect damage effected through autolysis/weakening of the cell wall and consequent osmotic lysis.

PMID: 15985350 [PubMed - indexed for MEDLINE]


7: Phytother Res. 2005 Mar;19(3):198-202.

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Antibacterial effect of plant extracts against Helicobacter pylori.

Nostro A, Cellini L, Di Bartolomeo S, Di Campli E, Grande R, Cannatelli MA, Marzio L, Alonzo V.

Pharmaco-Biological Department, University of Messina, Messina, Italy.

The aim of this work was to evaluate the antibacterial effect of plant extracts as alternative and[sol ]or as active agents supporting antibiotics for treating Helicobacter pylori infection. The effect of either, ethanolic or aqueous extracts from 17 plant materials were studied against one H. pylori standard strain and 11 clinical isolates using a disc diffusion test and by evaluating the minimum inhibitory concentration (MIC) on solid media. An inhibitory activity against H. pylori strains was recorded in a large percentage of tested plants. MIC values of ethanolic extracts were from two to four concentration steps lower than the aqueous ones. In particular, ethanolic extracts of Cuminum cyminum L. and Propolis expressed MIC90 values of 0.075 mg/mL. The results show a significant in vitro effect of plant extracts against H. pylori that could be considered a valuable support in the treatment of the infection and may contribute to the development of new and safe agents for inclusion in anti-H. pylori regimens. Copyright 2005 John Wiley & Sons, Ltd.

PMID: 15934015 [PubMed - indexed for MEDLINE]


8: Int J Gynaecol Obstet. 2005 May;89(2):127-32.

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Propolis solution for the treatment of chronic vaginitis.

Imhof M, Lipovac M, Kurz Ch, Barta J, Verhoeven HC, Huber JC.

Department of Obstetrics and Gynecology, AKH Vienna, University of Vienna, Vienna, Austria. martin.imhof@akh.ac.at

OBJECTIVE: To evaluate the efficacy of 5% propolis solution in recurrent vaginitis. METHOD: Fifty-four patients with recurrent vaginal infections having undergone at least one cycle of antibiotic treatment were instructed to apply a 5% aqueous propolis solution as a vaginal douche for seven days. Vaginal smears and specific symptoms were evaluated at baseline and 14 days after treatment. Long-term well-being was assessed by telephone interview six months after follow-up. RESULT: At the follow-up, the vaginal smears of 41 patients (75.9%) had improved. Forty-seven patients (87%) reported reliefs concerning at least one complaint. Associated improvement of smear and well-being was observed in 36 women (66.7%). After 6 months, 33 patients (61.1%) were satisfied with their condition without having undergone further treatment. CONCLUSION: Propolis may have a role as an alternative treatment for chronic vaginal infection.

Publication Types:

·       Clinical Trial


PMID: 15847875 [PubMed - indexed for MEDLINE]


9: Phytomedicine. 2005 Mar;12(3):221-8.

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Antibacterial activity of Turkish propolis and its qualitative and quantitative chemical composition.

Popova M, Silici S, Kaftanoglu O, Bankova V.

Institute of Organic Chemistry with Centre of Phytochemistry, Bulgarian Academy of Sciences, 1113 Sofia, Bulgaria.

The antibacterial activity of propolis from different regions of Turkey was studied, accompanied by TLC and GC-MS analyses of its chemical composition and spectrophotometric quantification of the most important active principles. All six samples were active against the bacterial test strains used; however, samples 1 (Yozgat), 2 (Izmir) and 3 (Kayseri) were more active than samples 4 (Adana), 5 (Erzurum) and 6 (Artvin). By TLC comparison all samples were found to contain poplar taxonomic markers but in samples 4 (Adana), 5 (Erzurum) and 6 (Artvin), different substances were observed, which were not present in P. nigra L. bud exudate. The typical poplar samples 1 (Yozgat), 2 (Izmir) and 3 (Kayseri) displayed very similar phenolic and flavonoid content. Samples 4 (Adana), 5 (Erzurum) and 6 (Artvin) were characterized by low phenolic and very low flavonoid concentrations. Qualitative analysis by GC-MS revealed that sample 4 (Adana) contained diterpenic acids and high percent of cinnamyl cinnamate, sample 5 (Erzurum)-significant amounts of hydroxy fatty acids and triterpenic alcohoLs, and sample 6 (Artvin)-phenolic glycerides, characteristic for the bud exudate of Populus euphratica Oliv. The results confirm the importance of phenolics for propolis antibacterial activity, and the significance of P. nigra L. as a propolis source, which provides the hive with the best defense against microorganisms.

PMID: 15830845 [PubMed - indexed for MEDLINE]


10: Contact Dermatitis. 2005 Feb;52(2):96-101.

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Rosacea and contact allergy to cosmetics and topical medicaments--retrospective analysis of multicentre surveillance data 1995-2002.

Jappe U, Schnuch A, Uter W.

Department of Dermatology, University of Heidelberg, Heidelberg, Germany. uta_jappe@med.uni-heidelberg.de

The role of contact allergy in rosacea has rarely been investigated. In this retrospective study, 361 out of 76,697 patients tested and documented by the Information Network of Departments of Dermatology between 1995 and 2002 had rosacea. Patch tests included standard series and constituents of cosmetics and topical medicaments. 118/361 had additionally been patch tested with their own cosmetics/medicaments. Positive reactions occurred to nickel (II) sulfate in 9.3%, fragrance mix in 8.8%, thimerosal in 6.9%, Myroxylon pereirae resin in 5.9%, potassium dichromate in 4.6% and propolis in 2.8%. Whereas rosacea patients had a significantly higher risk of contact allergy to propolis compared to the remaining patients, in an age- and sex-adjusted analysis, contact allergy to nickel was significantly less frequent in this group. For Lyral, the risk was elevated, albeit not significantly. Only 2/329 patients were positive to neomycin sulfate and 1/100 to gentamicin sulfate, among the panel of (topical) antibiotics tested. Among 118 patients tested with their own products, 3 were tested to metronidazole, 1 reacting positively. Irritant or doubtful patch test reactions were provoked by various substances (vehicles, oxidants and preservatives of various creams), which might also be clinically important, considering the heightened sensitivity of rosaceous skin.

Publication Types:

·       Multicenter Study


PMID: 15725288 [PubMed - indexed for MEDLINE]


11: J Agric Food Chem. 2005 Feb 23;53(4):1166-72.

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Some chemical composition and biological activity of northern Argentine propolis.

Isla MI, Paredes-Guzman JF, Nieva-Moreno MI, Koo H, Park YK.

Department of Food Science, College of Food Engineering, State University of Campinas, P.O. Box 6177, 13083-970, Campinas, Sao Paulo, Brazil.

Twenty-five samples of propolis were collected from seven different regions in northern Argentina; ethanolic extracts of propolis were prepared from all samples, and the respective samples were examined for UV absorption spectra, RPHPTLC, RPHPLC, antimicrobial activity, antiradical activity, and total phenolic content. It was found that 16 of the 25 samples showed a phenolic profile similar to that found in samples from southern Brazil and corresponding to poplar-based propolis and that the rest of the samples showed a different profile and higher antimicrobial and antiradical activities.

PMID: 15713035 [PubMed - indexed for MEDLINE]


12: Fitoterapia. 2004 Dec;75(7-8):683-9.

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New polyisoprenylated benzophenones from Venezuelan propolis.

Trusheva B, Popova M, Naydenski H, Tsvetkova I, Gregorio Rodriguez J, Bankova V.

Institute of Organic Chemistry with Centre of Phytochemistry, Bulgarian Academy of Sciences, 1113 Sofia, Bulgaria.

Two new polyisoprenylated benzophenones, 18-ethyloxy-17-hydroxy-17,18-dihydroscrobiculatone A and 18-ethyloxy-17-hydroxy-17,18-dihydroscrobiculatone B, together with the known scrobiculatones A and B, were isolated from Venezuelan propolis. The scrobiculatones A and B showed significant antibacterial activity and moderate toxicity to Artemia salina nauplii.

PMID: 15567244 [PubMed - indexed for MEDLINE]


13: FEMS Microbiol Lett. 2004 Nov 1;240(1):111-6.

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Galangin expresses bactericidal activity against multiple-resistant bacteria: MRSA, Enterococcus spp. and Pseudomonas aeruginosa.

Pepeljnjak S, Kosalec I.

Institute of Microbiology, Faculty of Pharmacy and Biochemistry, University of Zagreb, Schrottova 39/I, HR-10 000 Zagreb, Croatia.

The antimicrobial activity of three propolis ethanol extracts (EEP) was examined for various Gram-negative and Gram-positive bacterial species, including multiple-resistant Staphylococcus aureus, Enterococcus spp. and Pseudomonas aeruginosa strains. EEP had a good bactericidal activity against Gram-positive species, and all multiple-resistant bacterial strains tested were sensitive to EEP. Minimal inhibitory concentrations (MICs) were lower in samples of higher flavonoid content (from 0.65 to 7.81 mg mL(-1)), indicating the influence of the concentration of some potent bactericidal compound(s) in propolis or synergism among some bactericidal compounds. Antimicrobial-guided separation of flavonoid aglycones (bioassay in situ on thin-layer chromatogram) showed that galangin (3,5,7-trihydroxyflavone) is one compound in EEP with bactericidal activity. Galangin was isolated by preparative chromatography. After determining the quantity present, the MIC against multiple-resistant bacteria was determined. The MIC of galangin against multiple-resistant bacterial strains was significantly lower (from 0.16 to 0.44 mg mL(-1), p < 0.05) than that of EEP. The bactericidal activity of galangin against P. aeruginosa strains was present at 0.17+/-0.05 mg mL(-1).

PMID: 15500987 [PubMed - indexed for MEDLINE]


14: Pesqui Odontol Bras. 2004 Apr-Jun;18(2):174-9. Epub 2004 Aug 5.

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Natural medicaments in endodontics -- a comparative study of the anti-inflammatory action.

Silva FB, Almeida JM, Sousa SM.

Center of Biological Sciences and Professions of Health, University of Sacred Heart, Bauru.

The objective of this study was to evaluate the irritant potential of propolis, Casearia sylvestris, Otosporin and saline solution (control). Twenty-eight male Wistar rats were selected, anesthetized and four experimental sites were designed on their backs. Injections of 2% Evans blue were intravenously administered in the lateral caudal vein and 0.1 ml of the tested solutions was injected intradermally into the experimental sites. The animals were killed 1/2, 1, 3 and 6 hours after the injection of the solutions. Each piece of skin containing the lesion was immersed in formamide and incubated at 45 masculine C for 72 h. After filtration, optical density was measured in a spectrophotometer. Data were statistically analyzed by a 2-way non-parametric test. The highest values of extracted dye were observed at 3 hours characterizing a peak in the inflammatory process. Propolis was the least irritant solution. The natural medicaments tested in this study may be a valuable alternative for endodontic treatment.

Publication Types:

·       Clinical Trial


PMID: 15311323 [PubMed - indexed for MEDLINE]


15: Pharmacol Res. 2004 Mar;49(3):287-92.

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Effect of water extract of Turkish propolis on tuberculosis infection in guinea-pigs.

Yildirim Z, Hacievliyagil S, Kutlu NO, Aydin NE, Kurkcuoglu M, Iraz M, Durmaz R.

Department of Pulmonary Medicine, University of Inonu School of Medicine, Turgut Ozal Medical Centre, Kampus, 44069, Malatya, Turkey. zyildirim@inonu.edu.tr

Mycobacterium tuberculosis (H(37)R(v))-infected guinea-pig model was used to investigate the effect of water extract of propolis (WEP). After subcutaneous inoculation of tubercle bacilli, each animal received oral WEP (n=9), isoniazid (n=5) or saline (n=6) as placebo and were sacrificed 30 days later. Formation of necrosis was less prominent in the group treated with WEP, but was not statistically significant (P>0.05). The granuloma formation in the same group was more prominent than the placebo and isoniazid groups; however, this finding failed to reach statistical significance by the Kruskal-Wallis test (P>0.05). These findings suggest that Turkish WEP may have a limited effect on the development of tuberculosis infection in this guinea-pig model.

PMID: 14726226 [PubMed - indexed for MEDLINE]


16: Microbiol Res. 2003;158(4):353-7.

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In vitro antimicrobial activity of propolis and synergism between propolis and antimicrobial drugs.

Stepanovic S, Antic N, Dakic I, Svabic-Vlahovic M.

Department of Bacteriology, Institute of Microbiology and Immunology, School of Medicine, University of Belgrade, Dr Subotica 1, 11000 Belgrade, Serbia. stepan@afrodita.rcub.bg.ac.yu

The aim of this study was to investigate antimicrobial properties of ethanolic extract of 13 propolis (EEP) samples from different regions of Serbia against 39 microorganisms (14 resistant or multiresistant to antibiotics), and to determine synergistic activity between antimicrobials and propolis. Antimicrobial activity of propolis samples was evaluated by agar diffusion and agar dilution method. The synergistic action of propolis with antimicrobial drugs was assayed by the disc diffusion method on agar containing subinhibitory concentrations of propolis. Obtained results indicate that EEP, irrespectively of microbial resistance to antibiotics, showed significant antimicrobial activities against Gram-positive bacteria (MIC 0.078%-1.25% of EEP) and yeasts (0.16%-1.25%), while Gram-negative bacteria were less susceptible (1.25%-->5%). Enterococcus faecalis was the most resistant Gram-positive bacterium, Salmonella spp. the most resistant Gram-negative bacteria, and Candida albicans the most resistant yeast. EEP showed synergism with selected antibiotics, and displayed ability to enhance the activities of antifungals. The shown antimicrobial potential of propolis alone or in combination with certain antibiotics and antifungals is of potential medical interest.

PMID: 14717457 [PubMed - indexed for MEDLINE]


17: Clin Chim Acta. 2003 Dec;338(1-2):11-6.

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Protective role of Egyptian propolis against tumor in mice.

El-khawaga OA, Salem TA, Elshal MF.

Chemistry Department, Faculty of Science, Mansoura University, Mansoura City, Egypt. elkhawaga70s@mans.edu.eg

BACKGROUND: Propolis has numerous biologic activities including antibiotic, antifungal, antiviral and anti-inflammatory properties. The present work is aimed to study the effect of crude Egyptian propolis on tumor in mice induced by Ehrlich ascitis carcinoma (EAC) cell line. RESULTS: The administration of propolis (160 mg/kg body weight), by gastric intubation 2 h before the intraperitoneal injection of EAC, effectively inhibited tumor growth and the proliferation of EAC. The tumor volume was markedly reduced from 7+/-0.9 ml in EAC-infected mice to 1.6+/-0.95 ml in propolis-treated mice. Also, the lipid peroxide level which was 13.3+/-1.24 nmol malodialdehyde (MDA)/mg protein in EAC infected mice was significantly decreased to 3.3+/-2.1 nmol MDA/mg protein. Reduced glutathione (GSH) and glutathione S-transferase (GST) concentrations were markedly increased in propolis-treated mice. This effect was associated with inhibition of cell cycle progression and induction of apoptosis. Administration of propolis 2 h before injection of EAC arrested cells in G0/G1 phase and resulted in a decrease in the viability, DNA, total RNA and protein level of tumor cells. CONCLUSIONS: Crude Egyptian propolis has a strong inhibitory activity against tumors. The anti-tumor mechanism may be mediated by preventing oxidative damage and induction of apoptosis.

PMID: 14637260 [PubMed - indexed for MEDLINE]


18: J Ethnopharmacol. 2003 Oct;88(2-3):189-93.

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Flavonoids and trypanocidal activity of Bulgarian propolis.

Prytzyk E, Dantas AP, Salomao K, Pereira AS, Bankova VS, De Castro SL, Neto FR.

Ladetec, Instituto de Quimica, Universidade Federal do Rio de Janeiro, Ilha do Fundao, Cidade Universitaria, CT Bl.
A, 21949-900 Rio de Janeiro, RJ, Brazil.

Acetone and ethanol extracts of two Bulgarian propolis samples (Bur and Lov) were investigated by high temperature high resolution gas chromatography coupled to mass spectrometry (HT-HRGC-MS), and their activity against Trypanosoma cruzi was evaluated. The ethanol extracts--Et-Bur and Et-Lov--showed similar composition, with a high content of flavonoids, and strong inhibitory activity against T. cruzi proliferative epimastigotes, which were more susceptible than trypomastigotes. In the presence of blood, the activity of Et-Bur or Et-Lov against trypomastigotes was similar to that of the standard drug, crystal violet. Both extracts also showed similar and significant activity against Staphylococcus aureus and Candida albicans, while being inactive against Escherichia coli. The acetone extract, Ket-Bur, was more active than Et-Bur against both forms of T. cruzi.

PMID: 12963141 [PubMed - indexed for MEDLINE]


19: J Med Microbiol. 2003 May;52(Pt 5):417-9.

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Inhibition of Helicobacter pylori growth in vitro by Bulgarian propolis: preliminary report.

Boyanova L, Derejian S, Koumanova R, Katsarov N, Gergova G, Mitov I, Nikolov R, Krastev Z.

Department of Microbiology, Medical University of Sofia, Sofia, Bulgaria. l.boyanova@lycos.com

Bee glue (propolis) possesses antimicrobial, anti-inflammatory, anaesthetic and immunostimulating activities. The aim of the study was to evaluate the inhibitory effect of Bulgarian propolis on Helicobacter pylori growth in vitro. Activity of 30% ethanolic extract of propolis (EEP) against 38 clinical isolates of H. pylori was evaluated by using the agar-well diffusion method. Ethanol was used as a control. In addition, the effect of propolis on the growth of 26 H. pylori and 18 Campylobacter strains was tested by the disc diffusion method. Mean diameters of H. pylori growth inhibition by the agar-well diffusion method, using 30, 60 or 90 microl EEP or 30 microl ethanol per well, were 17.8, 21.2, 28.2 and 8.5 mm, respectively. EEP was significantly more active than ethanol against H. pylori (P < 0.001). The results obtained by the disc diffusion method were similar. The use of moist propolis discs resulted in mean diameters of growth inhibition of 21.4 mm for H. pylori and 13.6 mm for Campylobacter spp. Dried propolis discs exhibited antibacterial effect against 73.1% of H. pylori isolates, with a considerable zone of growth inhibition (> or = 15 mm) in 36.4% of isolates. Using dried propolis discs resulted in mean diameters of growth inhibition of 12.4 mm for H. pylori and 11.6 mm for Campylobacter spp. In conclusion, Bulgarian propolis possesses considerable antibacterial activity against H. pylori, and can also inhibit the growth of Campylobacter jejuni and Campylobacter coli. The potential of propolis in the prevention or treatment of H. pylori infection is worth further extensive evaluation.

PMID: 12721318 [PubMed - indexed for MEDLINE]


20: J Ethnopharmacol. 2003 May;86(1):69-73.

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Antimicrobial activity of propolis samples from two different regions of Anatolia.

Kartal M, Yildiz S, Kaya S, Kurucu S, Topcu G.

Department of Pharmacognosy, Faculty of Pharmacy, Ankara University, 06100 Ankara, Turkey. kartal@pharmacy.ankara.edu.tr

Antimicrobial activity of two propolis samples from Kazan and Marmaris regions in Turkey were investigated by the disc diffusion method. Antimicrobial activity was tested with four different ethanolic extracts (30, 50, 70, and 96% ethanol) of each sample against seven Gram positive, four Gram negative bacteria and one fungus culture. The activity was found to be mainly due to caffeic acid and its esters. An isomeric mixture containing 3,3-dimethylallyl caffeate, and isopent-3-enyl caffeate was isolated from Kazan propolis samples.

PMID: 12686444 [PubMed - indexed for MEDLINE]


21: Phytother Res. 2003 Mar;17(3):285-9.

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Brazilian propolis: physicochemical properties, plant origin and antibacterial activity on periodontopathogens.

Santos FA, Bastos EM, Maia AB, Uzeda M, Carvalho MA, Farias LM, Moreira ES.

Departamento de Microbiologia, Universidade Federal de Minas Gerais, Brazil.

Propolis samples collected in the dry and rainy seasons, from an experimental apiary located in a cerrado vegetation region in Brazil were used in this study. Microscopic analysis showed the presence of 31 pollen types, secretory hairs (genus Baccharis) and fragments of plant epidermis. The oxidation rates and the wax content of the samples after physicochemical analyses were in agreement with the Cuban Guideline NRAG 870-88. A high performance liquid chromatography analysis showed a similar pattern of chromatograms, characterized by the presence of ten phenolic compounds. There was no significant difference in the pro fi le of phenolic compounds and also in the total flavonoid concentration in propolis samples collected in different seasons. Antibacterial assays were performed by the method of dilution of an ethanol extract of propolis (EEP) in agar (v/v%) and showed that all 16 A. actinomycetemcomitans strains tested were inhibited by propolis concentrations of 0.1% to 0.25%, and did not grow at all at 0.5%. The growth inhibition of six Fusobacterium spp. and 16 black-pigmented anaerobes was observed at concentrations of 0.05% to 0.1%, and no growth was observed at 0.25%. There was no effect of seasonality on the inhibitory activity of propolis. The antibiotics tetracycline and meropenem were used as positive controls. Copyright 2003 John Wiley & Sons, Ltd.

PMID: 12672164 [PubMed - indexed for MEDLINE]


22: Pol J Vet Sci. 2002;5(4):223-5.

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Investigation on the inhibition of Campylobacter jejuni growth with the applications of some preservatives, medicines, herbs and herb preparations.

Uradzinski J, Szteyn J, Kafel S.

Department of Animal Products Hygiene, Faculty of Veterinary Medicine, University of Warmia and Mazury, Oczapowskiego 14, 10-719 Olsztyn, Poland. janurad@moskit.uwm.edu.pl

The aim of this study was to determine the influence of chosen preservatives, medicines, herbs and herb preparations on 39 Campylobacter jejuni strains, which were isolated from fresh poultry carcasses. De-Nol and ventrisol were used in the original concentration. Propolis, propolan, artecholin and aukalen were of pharmacies origin. The rest of substances examined were 10% water solutions. Out of 11 substances examined, potassium sorbate, sodium benzoate, De-Nol and ventrisol exerted the strongest inhibitory action on Campylobacter jejuni.

PMID: 12512554 [PubMed - indexed for MEDLINE]


23: Fitoterapia. 2002 Nov;73 Suppl 1:S1-6.

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Propolis, an old remedy used in modern medicine.

Castaldo S, Capasso F.

Department of I.N.P.S. of Naples, via Medina 63, Naples, Italy.

Propolis is one of the few natural remedies that has maintained its popularity over a long period of time. The pharmacologically active molecules in the propolis are flavonoids and phenolic acids and their esters. These components have multiple effects on bacteria, fungi and viruses. In addition, propolis and its components have anti-inflammatory and immunomodulatory activities. Moreover, propolis has been shown to lower blood pressure and cholesterol levels. However, clinical studies to substantiate these claims are required.

Publication Types:

·       Review


PMID: 12495704 [PubMed - indexed for MEDLINE]


24: Pol J Vet Sci. 2002;5(3):197-202.

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The use of some immunomodulators and non-antibiotic drugs in a prophylaxis and treatment of mastitis.

Malinowski E.

Department of Pathophysiology of Reproduction and Mammary Gland. National Veterinary Research Institute, Powstancow Wikp, 10, 85-090 Bydgoszcz, Poland.

Antibiotics are widely used in treatment and prophylaxis of mastitis, but their antibacterial and therapeutic efficacy continues to decrease. New methods of prophylaxis of mastitis with the use of vaccines and biological response modifiers are reviewed. Prophylactic activity of staphylococcal vaccines should be investigated in the future because of difficulties in successful treatment of infections caused by these organisms. Vaccination with J5 bacterin (0111:B4) reduced duration of IMI and local signs of clinical coliform-mastitis. Some immunomodulators proved to be effective in treatment of mastitis during lactating period. Promising results were obtained with the use of giseng saponin, herbal gel, herbal extracts, propolis, lysosubtilin, antibacterial proteins, and lysozyme dimer especially.

Publication Types:

·       Review


PMID: 12448082 [PubMed - indexed for MEDLINE]


25: Z Naturforsch [C]. 2002 Mar-Apr;57(3-4):395-402.

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Egyptian propolis: 3. Antioxidant, antimicrobial activities and chemical composition of propolis from reclaimed lands.

Hegazi AG, Abd El Hady FK.

Department of Parasitology, National Research Center, Dokki, Giza, Egypt. ahmedgaffer@mailer.suc.eun.eg

The free radical scavenging effect of two propolis samples collected from reclaimed land, Egypt as well as of vitamin C and caffeic acid in 1,1-diphenyl-2-picrylhydrazyl (DPPH) free radical system was determined. The antimicrobial (Staphylococcus aureus; Escherichia coli and Candida albicans) activity was also investigated. The results of the free radical scavenging effect of El-Saff and Ismailia propolis showed a concentration-dependent activity. The antioxidant activity was varied according to the examined material. It was obvious that caffeic acid and vitamin C showed the highest activity if compared with the propolis samples. El- Saff propolis had a higher antioxidant activity than Ismailia propolis, it showed a higher antibacterial activity against Staphylococcus aureus and a higher anti-fungal activity against Candida albicans. While the Ismailia propolis had a higher antibacterial activity against Escherichia coli, than El-Saff propolis. The chemical composition of propolis samples was investigated by GC/MS, where 75 compounds were identified, 22 being new for propolis. The Ismailia propolis was characterized by the presence of a highly significant amount of aromatic acid esters (47.3%) and triterpenoids (17.3%), while El-Saff propolis contained 3% and 1.9% respectively. The new esters belonged to 4-methoxyhydrocinnamic acid, hydroferulic acid and ferulic acid. El-Saff propolis had a very high significant amount (27%) of 2,6-bis-(pentanyloxy)-4-pentanylphenethanol, which is also a new compound for propolis.

PMID: 12064746 [PubMed - indexed for MEDLINE]


26: Z Naturforsch [C]. 2002 Mar-Apr;57(3-4):386-94.

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Egyptian propolis: 2. Chemical composition, antiviral and antimicrobial activities of East Nile Delta propolis.

Abd El Hady FK, Hegazi AG.

Department of Chemistry of Natural Products, National Research Center, Dokki, Giza, Egypt.

Three propolis samples from East Nile Delta, Egypt were collected. Propolis samples were investigated by GC/MS,103 compounds were identified, 20 being new for propolis. Dakahlia propolis was a typical poplar propolis but it contained two new caffeate esters and two new triterpenoids. Ismailia propolis was characterized by the presence of new triterpenic acid methyl esters and it did not contain any aromatic acids, esters and flavonoids. Sharkia propolis was characterized by the presence of caffeate esters only, some di- and triterpenoids. The antiviral (Infectious Bursal Disease Virus and Reo-Vi